| Resistance to apoptosis is correlated with the reduced caspase-3 activation and enhanced expression of antiapoptotic proteins in human cervical multidrug-resistant cells. | |
| | |
MedLine Citation:
|
PMID: 10753639 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Recent studies have indicated that induction of apoptosis is the primary cytotoxic mechanism of most cancer chemotherapeutic agents, and abnormalities in the control of apoptosis can affect the sensitivity of malignant cells to multiple drugs. Here, we treated cells with cisplatin and other apoptotic stimuli and found that multidrug-resistant (MDR) endocervical HEN-16-2/CDDP cells, compared with drug-sensitive parental cells, were significantly more resistant to apoptosis and exhibited decreased proteolytic activation of caspase-3. The latter was further demonstrated by decreased cleavage of its substrate poly(ADP-ribose) polymerase (PARP). Further, Western blot analysis showed that MDR HEN-16-2/CDDP cells had significantly higher levels of the apoptosis-inhibiting proteins BAG-1 p50 and p33 isoforms and Bcl-X(L). This study provided the first evidence that overexpression of antiapoptotic BAG-1 p50 and p33 and Bcl-X(L) may cause resistance to apoptosis through reduction of caspase-3 activity in human cervical cells having an MDR phenotype. |
| | |
Authors:
|
Z Ding; X Yang; A Pater; S C Tang |
Related Documents
:
|
8637709 - Bcl-2 and adenovirus e1b 19 kda protein prevent e1a-induced processing of cpp32 and cle... 17606599 - Coxiella burnetii inhibits apoptosis in human thp-1 cells and monkey primary alveolar m... 14715469 - Oxidative stress induces apoptosis in c6 glioma cells: involvement of mitogen-activated... 19900759 - Tuning of apoptosis-mediator gene transcription in hepg2 human hepatoma cells through a... 18678619 - Nonsteroidal anti-inflammatory drugs induced endothelial apoptosis by perturbing peroxi... 11526489 - Ionizing radiation but not anticancer drugs causes cell cycle arrest and failure to act... 11359809 - Inhibition of p38 kinase reveals a tnf-alpha-mediated, caspase-dependent, apoptotic dea... 18041089 - The peroxisome proliferator-activated receptor-gamma agonist 1,1-bis(3'-indolyl)-1-(p-t... 19304799 - Hla-g homodimer-induced cytokine secretion through hla-g receptors on human decidual ma... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 270 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2000 Apr |
Date Detail:
|
Created Date: 2000-05-08 Completed Date: 2000-05-08 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 415-20 Citation Subset: IM |
Copyright Information:
|
Copyright 2000 Academic Press. |
Affiliation:
|
Division of Basic Medical Sciences, Memorial University of Newfoundland, St. John's, Newfoundland, A1B 3V6, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetyltransferases* Apoptosis* Carrier Proteins / metabolism* Caspase 3 Caspases / metabolism* Cell Line, Transformed Cervix Uteri / cytology, enzymology, metabolism* Cisplatin / pharmacology DNA-Binding Proteins Drug Resistance, Multiple* Enzyme Activation Female Humans Proteins / metabolism* Proto-Oncogene Proteins c-bcl-2 / metabolism* Transcription Factors bcl-X Protein |
| Chemical | |
Reg. No./Substance:
|
0/BCL2-associated athanogene 1 protein; 0/BCL2L1 protein, human; 0/Carrier Proteins; 0/DNA-Binding Proteins; 0/Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Transcription Factors; 0/bcl-X Protein; 15663-27-1/Cisplatin; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/mim-1 protein, Gallus gallus; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Induction of apoptosis and mitosis inhibition by degraded DNA lipotransfection mimicking genotoxic d...
Next Document: Plicatamide: A lead to the biosynthetic origins of the tunichromes?