Document Detail

Resistance of neonatal primary astrocytes against Fas-induced apoptosis depends on silencing of caspase 8.
MedLine Citation:
PMID:  20510339     Owner:  NLM     Status:  MEDLINE    
In the present report, we have found that primary fetal astrocytes express caspase 8 and undergo apoptosis in response to Fas ligation. In contrast, neonatal astrocytes do not express detectable levels of the enzyme and are resistant to Fas killing. Fas-induced apoptosis can be restored in these cells by up-regulation of caspase 8 expression by means of transient transfection with a caspase 8-encoding plasmid. Furthermore, treatment of primary astrocytes with the demethylating agent 5-Aza-dC restores caspase 8 expression and increases the sensibility of neonatal astrocytes to the cytotoxic effect of Fas activation. Altogether, our findings indicate that silencing of caspase 8 gene is a key factor controlling the outcome of neonatal astrocytes upon Fas engagement.
Olga Barca; Carmen Carneiro; José A Costoya; Rosa M Señarís; Víctor M Arce
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-25
Journal Detail:
Title:  Neuroscience letters     Volume:  479     ISSN:  1872-7972     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-12     Completed Date:  2010-09-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  206-10     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
Departamento de Fisioloxía, Facultade de Medicina, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
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MeSH Terms
Animals, Newborn
Astrocytes / cytology*
Caspase 8 / biosynthesis,  genetics*
Cerebral Cortex / cytology
Gene Silencing
Rats, Sprague-Dawley
Reg. No./Substance:
EC 3.4.22.-/Caspase 8

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