Document Detail


Resistance of melanized yeast cells of Paracoccidioides brasiliensis to antimicrobial oxidants and inhibition of phagocytosis using carbohydrates and monoclonal antibody to CD18.
MedLine Citation:
PMID:  19722091     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Paracoccidioides brasiliensis, a thermal dimorphic fungal pathogen, produces a melanin-like pigment in vitro and in vivo. We investigated the involvement of carbohydrates and monoclonal antibody to CD18, on phagocytosis inhibition, involving macrophage receptors and the resistance of melanized fungal cells to chemically generated nitric oxide (NO), reactive oxygen species (ROS), hypochlorite and H2O2. Our results demonstrate that melanized yeast cells were more resistant than nonmelanized yeast cells to chemically generated NO, ROS, hypochlorite and H2O2, in vitro. Phagocytosis of melanized yeast cells was virtually abolished when mannan, N-acetyl glucosamine and anti-CD18 antibody were added together in this system. Intratracheal infection of BALB/c mice, with melanized yeast cells, resulted in higher lung colony forming units, when compared to nonmelanized yeast cells. Therefore, melanin is a virulence factor of P. brasiliensis.
Authors:
Marcelo Barbosa da Silva; Luciana Thomaz; Alexandre Ferreira Marques; Artur E Svidzinski; Josh D Nosanchuk; Arturo Casadevall; Luiz R Travassos; Carlos P Taborda
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Memórias do Instituto Oswaldo Cruz     Volume:  104     ISSN:  1678-8060     ISO Abbreviation:  Mem. Inst. Oswaldo Cruz     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-09-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502619     Medline TA:  Mem Inst Oswaldo Cruz     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  644-8     Citation Subset:  IM    
Affiliation:
Departamento de Microbiologia, Laboratório de Micologia Médica - LIM53 - Hospital de Clínicas, Faculdade de Medicina, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brasil .
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Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
AI056070-01A2/AI/NIAID NIH HHS

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