Document Detail

Resistance of hamster bronchiolar epithelium to neutrophil elastase: investigation by cell surface lectin cytochemistry.
MedLine Citation:
PMID:  1572319     Owner:  NLM     Status:  MEDLINE    
An intratracheal instillation of human neutrophil elastase (HNE) causes accumulation of an excess number of secretory granules in the epithelial secretory cells lining the hamster bronchus. This chronic lesion, which we refer to as secretory cell metaplasia (SCM), is not seen in the trachea or bronchioles. Because luminal cell surface lectin binding is much higher in the trachea than in the bronchus, we concluded that tracheal resistance may be due to a protective glycoconjugate coat. In the present ultrastructural study, we analyzed the lectin-binding capability of bronchiolar epithelial cells to determine whether their luminal cell surface glycoconjugate layer is similar to tracheal epithelial cells. None of the six ferritin-conjugated lectins showed higher binding in bronchioles compared to the bronchus, suggesting that a high level of surface oligosaccharides is not necessary for resistance to the metaplastic effects of HNE. HNE caused a significant reduction in bronchiolar surface binding of the gold-labeled, secretory cell-specific lectin, Helix pomatia agglutinin. The principal granulated secretory cell type in bronchioles was ultrastructurally similar to a form of bronchial Clara cell that converts to a mucous cell phenotype in response to HNE. The results suggest that absence of bronchiolar SCM is not attributable to a protective layer of cell surface oligosaccharides, a lack of cellular contact by HNE, or the presence of a morphologically distinct population of epithelial cells in bronchioles.
T G Christensen; R Breuer; C E Haddad; E C Lucey; P J Stone; G L Snider
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental lung research     Volume:  18     ISSN:  0190-2148     ISO Abbreviation:  Exp. Lung Res.     Publication Date:    1992 Jan-Mar
Date Detail:
Created Date:  1992-06-04     Completed Date:  1992-06-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8004944     Medline TA:  Exp Lung Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  115-29     Citation Subset:  IM    
Mallory Institute of Pathology, Boston, MA 02118.
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MeSH Terms
Bronchi / metabolism*
Cell Membrane / metabolism
Epithelium / metabolism
Helix (Snails)*
Instillation, Drug
Intubation, Intratracheal
Neutrophils / enzymology*
Oligosaccharides / metabolism*
Pancreatic Elastase / metabolism*
Grant Support
Reg. No./Substance:
0/Helix lectin; 0/Lectins; 0/Oligosaccharides; EC Elastase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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