Document Detail


Resistance exercise increases postprandial muscle protein synthesis in humans.
MedLine Citation:
PMID:  19092695     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: We examined the impact of an acute bout of resistance-type exercise on mixed muscle protein synthesis in the fed state. METHODS: After a standardized breakfast, 10 untrained males completed a single, unilateral lower-limb resistance-type exercise session. A primed, continuous infusion of l-[ring-C6]phenylalanine was combined with muscle biopsy collection from both the exercised (Ex) and the nonexercised (NEx) leg to assess the impact of local muscle contractions on muscle protein synthesis rates after food intake. Western blotting with phosphospecific and pan antibodies was used to determine the phosphorylation status of AMP-activated kinase (AMPK), 4E-binding protein (4E-BP1), mammalian target of rapamycin (mTOR), and p70 ribosomal protein S6 kinase (S6K1). RESULTS: Muscle protein synthesis rates were approximately 20% higher in Ex compared with NEx (0.098% +/- 0.005% vs 0.083% +/- 0.002%.h, respectively, P < 0.01). In the fed state, resistance-type exercise did not elevate AMPK phosphorylation. However, the phosphorylation status of 4E-BP1 was approximately 20% lower after cessation of exercise in Ex compared with NEx (P < 0.05). Conversely, 4E-BP1 phosphorylation was significantly higher in Ex compared with NEx after 6 h of recovery (P < 0.05) with no changes in mTOR phosphorylation. S6 phosphorylation was greater in Ex versus NEx after cessation of exercise (P < 0.05), although S6K1 phosphorylation at T was not up-regulated (P > 0.05). CONCLUSION: We conclude that resistance-type exercise performed in a fed state further elevates postprandial muscle protein synthesis rates, which is accompanied by an increase in S6 and 4E-BP1 phosphorylation state.
Authors:
Oliver C Witard; Michael Tieland; Milou Beelen; Kevin D Tipton; Luc J C van Loon; René Koopman
Related Documents :
20507965 - Expiratory muscle loading increases intercostal muscle blood flow during leg exercise i...
15640275 - Exercise-induced metallothionein expression in human skeletal muscle fibres.
2383805 - A model for nonexercising hindlimb muscles in exercising animals.
8007805 - Influence of age on skeletal muscle glucose transport and glycogen metabolism.
7474535 - The effects of mild, moderate, and severe exercise on intraocular pressure in glaucoma ...
7474995 - The concept of lactate threshold. a short review.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medicine and science in sports and exercise     Volume:  41     ISSN:  1530-0315     ISO Abbreviation:  Med Sci Sports Exerc     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-24     Completed Date:  2009-05-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8005433     Medline TA:  Med Sci Sports Exerc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  144-54     Citation Subset:  IM; S    
Affiliation:
School of Sport and Exercise Sciences, University of Birmingham, Birmingham, UNITED KINGDOM.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Biopsy
Body Composition
Humans
Male
Muscle Contraction / physiology*
Muscle, Skeletal / metabolism,  physiology*
Postprandial Period*
Prokaryotic Initiation Factor-3
Prospective Studies
Protein Kinases
Resistance Training*
Chemical
Reg. No./Substance:
0/Prokaryotic Initiation Factor-3; EC 2.7.-/Protein Kinases; EC 2.7.1.-/mTOR protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Protein modification responds to exercise intensity and antioxidant supplementation.
Next Document:  Plasma visfatin and ghrelin response to prolonged sculling in competitive male rowers.