Document Detail

Resistance determinants in strains of Clostridium difficile from two geographically distinct populations.
MedLine Citation:
PMID:  15555889     Owner:  NLM     Status:  MEDLINE    
Ninety-three clinical isolates of Clostridium difficile, comprising 65 from Royal Gwent Hospital, Newport and 28 from Southmead Hospital, Bristol were examined to determine the prevalence of genes coding for macrolide resistance and to explore differences in susceptibility patterns. Antibiogram testing produced similar results for both sets of strains with respect to amoxicillin, tetracycline, erythromycin and cefotaxime. Results differed for rifampicin, where 53% of the Bristol isolates were resistant, compared with 3% of the Newport isolates. Clindamycin disc susceptibility testing produced similar resistance rates. However, clindamycin MIC determinations revealed that 53% of the Bristol strains exhibited high-level resistance (MIC > 256 mg/L), whereas strains from Newport had clindamycin MICs ranging from 0.25 to 3mg/L. erm (B) was present in 15 of the strains from Bristol and in none of the Newport strains. erm (F) and erm (Q) were not detected in either population. The two geographically distinct populations of C. difficile differed considerably in their susceptibility to antibiotics. The possibility that C. difficile may serve as a conservator for resistant determinants subsequent to exposure to antimicrobial agents, has important implications for infection control.
Julian S Bendle; Peter A James; Peter M Bennett; Matthew B Avison; Alasdair P Macgowan; Khalid M Al-Shafi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of antimicrobial agents     Volume:  24     ISSN:  0924-8579     ISO Abbreviation:  Int. J. Antimicrob. Agents     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-23     Completed Date:  2005-04-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9111860     Medline TA:  Int J Antimicrob Agents     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  619-21     Citation Subset:  IM    
Department of Microbiology, Royal Gwent Hospital, Gwent Healthcare NHS Trust, Cardiff Road, Newport, South Wales NP20 2UB, UK.
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MeSH Terms
Bacterial Proteins
Cephalosporins / pharmacology*
Clostridium difficile / drug effects*,  genetics
Drug Resistance, Bacterial*
Genes, Bacterial
Microbial Sensitivity Tests
beta-Lactams / pharmacology*
Reg. No./Substance:
0/Bacterial Proteins; 0/Cephalosporins; 0/beta-Lactams; EC 2.1.1.-/Methyltransferases; EC protein, Bacteria

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