| Resistance carrying plasmid in a traumatic wound. | |
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MedLine Citation:
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PMID: 20616773 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To isolate and identify antibiotic-resistant bacteria from the exudate of a complex wound and determine if antibiotic resistance genes are chromosomal or plasmid borne. METHOD: Antibiotic resistant bacteria from wound exudate of a single clinical sample were selected on agar media with ampicillin. A single colony was further screened for resistance to kanamycin by antibiotic-supplemented agar and to other antibiotics by an automated Phoenix instrument. Identification of the isolate was carried out by biochemical profiling and by 16S rDNA analysis. RESULTS: Approximately 51% of total bacteria in the wound exudate with identical colony morphotype were resistant to 100 microg/ml of ampicillin. A single colony from this population also demonstrated resistance to 50 microg/ml of kanamycin on kanamycin-supplemented agar. Further antimicrobial sensitivity testing by the Phoenix instrument indicated resistance to inhibitory concentrations of amoxicillin-clavulanate, ampicillin-sulbactam, cefazolin, gentamicin, nitrofurantoin, tobramycin, and trimethoprim-sulfamethoxazole. Biochemical and 16S rDNA analysis identified this bacterial isolate as a member of genus Enterobacter. A plasmid preparation from this isolate successfully transferred ampicillin and kanamycin resistance to E. coli competent cells. E. coli transformants displayed two resistance phenotypes and the plasmids from these transformants displayed two different restriction type patterns, with one correlating to ampicillin and kanamycin resistance and the other only to ampicillin resistance. CONCLUSION: A multiple antibiotic-resistant Enterobacter spp. from the wound fluid of a clinical sample was found to carry an antibiotic-resistant plasmid in a closely related species E. coli. The presence of antibiotic resistance plasmid in Enterobacteria that are part of the normal microbial flora of the human gut and skin could lead to the spread of resistance phenotype and emergence of antibiotic resistant pathogens. This study suggests normal human microbial fl ora could be a potential reservoir for resistance genes. |
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Authors:
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M R Alavi; A Ravizee; R Burgess; V Antonic; M Izadjoo; A Stojadinovic |
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Publication Detail:
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Type: Case Reports; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of wound care Volume: 19 ISSN: 0969-0700 ISO Abbreviation: J Wound Care Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-09 Completed Date: 2010-09-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9417080 Medline TA: J Wound Care Country: England |
Other Details:
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Languages: eng Pagination: 306-10 Citation Subset: N |
Affiliation:
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Wound Biology and Translational Research Division, Armed Forces Institute of Pathology, Washington DC, USA. alavim@afip.osd.mil |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Typing Techniques DNA, Bacterial / analysis, genetics Electrophoresis, Agar Gel Enterobacter / classification, genetics* Enterobacteriaceae Infections / microbiology* Exudates and Transudates / microbiology* Feasibility Studies Humans Kanamycin Resistance / genetics* Mass Screening / methods Microbial Sensitivity Tests Phylogeny Plasmids / genetics* Polymerase Chain Reaction Sequence Alignment Sequence Analysis, DNA Wound Infection / microbiology* Wounds, Penetrating / complications |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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