Document Detail


Resistance of Sprague-Dawley Rats to infection with Helicobacter pullorum.
MedLine Citation:
PMID:  23294887     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Helicobacter pullorum is an enterohepatic Helicobacter spp. known to infect both chickens and humans. H. pullorum infection has recently been reported in both mice and rats. This study was designed to determine whether standard methods of colony health surveillance using exposure to rodent soiled bedding could detect H. pullorum in Sprague-Dawley rats. We exposed 8 Helicobacter-free Sprague-Dawley rats to bedding from H. pullorum-infected Brown Norway rats. Fecal samples were analyzed by PCR every 2 wk for 22 wk. Dirty bedding transfer resulted in intermittent positive fecal PCR results; however, none of the rats became persistently infected with H. pullorum. Select intestinal tissues collected at necropsy analyzed by PCR were negative for H. pullorum. To determine whether the failure to detect H. pullorum in Sprague-Dawley rats receiving contact bedding was due to resistance of Sprague-Dawley rats to H. pullorum colonization, 10 Helicobacter-free Sprague-Dawley rats were orally dosed with H. pullorum. Fecal samples were analyzed by PCR every 2 wk for 12 wk. At 2 wk after infection, 5 of 10 rats were PCR positive for H. pullorum. By 12 wpi, only 2 rats were persistently colonized with H. pullorum according to culture and PCR results. These data contrast with our previous data, which showed a high frequency of both natural and experimental H. pullorum infection in Brown Norway rats. Sprague-Dawley rats are resistant to experimentally induced H. pullorum gastrointestinal colonization when dosed orally with H. pullorum or exposed to bedding from H. pullorum-infected rats.
Authors:
Laura D Cacioppo; Zeli Shen; Nicola M Parry; James G Fox
Related Documents :
25069887 - Apple polyphenol extracts protect against aspirin-induced gastric mucosal damage in rats.
17010607 - Xanthine mimetics as potent dipeptidyl peptidase iv inhibitors.
23258997 - Effects of moxibustion stimulation on the intensity of infrared radiation of tianshu (s...
3481337 - Naloxone-reversible antidiarrheal effects of enkephalinase inhibitors.
1762077 - Species variation in pulmonary endothelial aminopeptidase p activity.
12191677 - Effect of ursodeoxycholate-3,7-disulfate on biliary excretion of lithocholate-3-o-glucu...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of the American Association for Laboratory Animal Science : JAALAS     Volume:  51     ISSN:  1559-6109     ISO Abbreviation:  J. Am. Assoc. Lab. Anim. Sci.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2013-01-08     Completed Date:  2013-09-20     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  101269489     Medline TA:  J Am Assoc Lab Anim Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  803-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Female
Helicobacter / classification,  physiology*
Helicobacter Infections / immunology,  microbiology,  veterinary*
Housing, Animal
Male
Rats
Rats, Sprague-Dawley*
Rodent Diseases / immunology*,  microbiology,  transmission
Grant Support
ID/Acronym/Agency:
P30 ES002109/ES/NIEHS NIH HHS; P30-ES02109/ES/NIEHS NIH HHS; R01AI50952/AI/NIAID NIH HHS; R01RR032307/RR/NCRR NIH HHS; T32RR070036/RR/NCRR NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Transmission of mouse parvovirus to neonatal mice.
Next Document:  Evaluation of common anesthetic and analgesic techniques for tail biopsy in mice.