| Resin characterization by electro-acoustic measurements. | |
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MedLine Citation:
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PMID: 17194462 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The electro-acoustic effects, namely the ion vibration potential (IVP) and the colloidal vibration current (CVI), colloidal vibration potential (CVP) first described by P. Debye [P. Debye, J. Chem. Phys. 1 (1933) 13], are a result of charge separation of bound or free ions at different degrees by ultrasonic waves. Today commercial instruments are available to investigate liquid homogeneous and heterogeneous systems. In the present paper the application of this technique for the characterization of salts, protein solutions and resins for biochromatography is shown and valuable information about resins can be derived in a short time. Various resins were investigated with the following results: (1) the CVI magnitude is dependent of several parameters (such as particle size distribution, volume fraction, density difference); (2) the CVI is influenced by the surface modification of the resins. Polymeric modifications decrease the value of CVI. The CVI is generally lower for high capacity resins; (3) the measurement of the electro-acoustic effects can be used to detect small changes in resins. The CVI is dependent of the amount of adsorbed protein in "native" and denatured state. |
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Authors:
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Egbert Müller; Christian Mann |
Publication Detail:
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Type: Journal Article Date: 2006-12-27 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1144 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-26 Completed Date: 2007-05-21 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 30-9 Citation Subset: IM |
Affiliation:
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Tosoh Bioscience GmbH, Zettachring 6, 70567 Stuttgart, Germany. egbert.mueller@tosohbioscience.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acoustics Chromatography / instrumentation, methods* Ion Exchange Resins / analysis*, chemistry Models, Theoretical Nanoparticles Particle Size Vibration* |
| Chemical | |
Reg. No./Substance:
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0/Ion Exchange Resins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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