Document Detail

Residues in the pore region of Drosophila transient receptor potential A1 dictate sensitivity to thermal stimuli.
MedLine Citation:
PMID:  23027824     Owner:  NLM     Status:  MEDLINE    
The capacity to sense temperature is essential for the survival of all animals. At the molecular level, ion channels belonging to the transient receptor potential (TRP) family of channels function as temperature sensors in animals across several phyla. TRP channels are opened directly by changes in temperature and show pronounced sensitivity at their activation range. To determine how temperature activates these channels, we analysed channels belonging to the TRPA family, which detect heat in insects and cold in mammals. By constructing chimeric proteins consisting of human and Drosophila TRPA1 channels, we mapped regions that regulate thermal activation and identified residues in the pore helix that invert temperature sensitivity of TRPA1. From analysis of individual channels we defined the gating reaction of Drosophila TRPA1 and determined how mutagenesis alters the energy landscape for channel opening. Our results reveal specific molecular requirements for thermal activation of TRPA1 and provide mechanistic insight into this process.
Hong Wang; Melanie Schupp; Sandra Zurborg; Paul A Heppenstall
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-01
Journal Detail:
Title:  The Journal of physiology     Volume:  591     ISSN:  1469-7793     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-02     Completed Date:  2013-06-13     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  185-201     Citation Subset:  IM    
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MeSH Terms
Calcium Channels / chemistry,  physiology*
Drosophila Proteins / chemistry,  physiology*
Hot Temperature
Nerve Tissue Proteins / chemistry,  physiology*
TRPC Cation Channels / chemistry,  physiology*
Transient Receptor Potential Channels / chemistry,  physiology*
Reg. No./Substance:
0/Calcium Channels; 0/Drosophila Proteins; 0/Nerve Tissue Proteins; 0/TRPA1 protein, human; 0/TRPC Cation Channels; 0/Transient Receptor Potential Channels; 0/TrpA1 protein, Drosophila

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