| Residues in the TATA-binding protein required to mediate a transcriptional response to retinoic acid in EC cells. | |
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MedLine Citation:
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PMID: 8413615 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The eukaryotic TATA-binding protein TBP, which is required for transcription by RNA polymerase II, is tightly associated with a particular set of factors in the TFIID complex, and as such provides a target for transcriptional regulation exerted by upstream factors. An embryonic carcinoma (EC) cell-specific activity like that of the viral factor E1A has been implicated in the mediation of transactivation from the retinoic acid receptor to human TBP, but yeast TBP cannot perform this function. Using TBP mutants with an altered TATA-box-binding specificity, we show here that yeast TBP can mediate transcriptional activation in mammalian cells and that its inability to convey retinoic acid-dependent transactivation in EC cells is due to specific residues in its core region. These residues preclude a functional association with the cellular E1A-like activity. TBP is thus a target for retinoic acid-dependent transactivation in EC cells by providing a surface for interaction with the EC cell-specific E1A-like activity. |
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Authors:
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M Keaveney; A Berkenstam; M Feigenbutz; G Vriend; H G Stunnenberg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nature Volume: 365 ISSN: 0028-0836 ISO Abbreviation: Nature Publication Date: 1993 Oct |
Date Detail:
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Created Date: 1993-11-09 Completed Date: 1993-11-09 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0410462 Medline TA: Nature Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 562-6 Citation Subset: IM |
Affiliation:
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Gene Expression Program, EMBL, Heidelberg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenovirus E1A Proteins
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metabolism Amino Acid Sequence Base Sequence Binding Sites Cell Line, Transformed Computer Graphics DNA Humans Molecular Sequence Data Promoter Regions, Genetic Protein Conformation Receptors, Retinoic Acid / genetics, metabolism Recombinant Fusion Proteins / metabolism Saccharomyces cerevisiae Transcription Factor TFIID Transcription Factors / chemistry, metabolism* Transcription, Genetic* Transcriptional Activation Transfection Tretinoin / pharmacology* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Adenovirus E1A Proteins; 0/Receptors, Retinoic Acid; 0/Recombinant Fusion Proteins; 0/Transcription Factor TFIID; 0/Transcription Factors; 302-79-4/Tretinoin; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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