Document Detail


Residues in the TATA-binding protein required to mediate a transcriptional response to retinoic acid in EC cells.
MedLine Citation:
PMID:  8413615     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The eukaryotic TATA-binding protein TBP, which is required for transcription by RNA polymerase II, is tightly associated with a particular set of factors in the TFIID complex, and as such provides a target for transcriptional regulation exerted by upstream factors. An embryonic carcinoma (EC) cell-specific activity like that of the viral factor E1A has been implicated in the mediation of transactivation from the retinoic acid receptor to human TBP, but yeast TBP cannot perform this function. Using TBP mutants with an altered TATA-box-binding specificity, we show here that yeast TBP can mediate transcriptional activation in mammalian cells and that its inability to convey retinoic acid-dependent transactivation in EC cells is due to specific residues in its core region. These residues preclude a functional association with the cellular E1A-like activity. TBP is thus a target for retinoic acid-dependent transactivation in EC cells by providing a surface for interaction with the EC cell-specific E1A-like activity.
Authors:
M Keaveney; A Berkenstam; M Feigenbutz; G Vriend; H G Stunnenberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nature     Volume:  365     ISSN:  0028-0836     ISO Abbreviation:  Nature     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1993-11-09     Completed Date:  1993-11-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0410462     Medline TA:  Nature     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  562-6     Citation Subset:  IM    
Affiliation:
Gene Expression Program, EMBL, Heidelberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adenovirus E1A Proteins / metabolism
Amino Acid Sequence
Base Sequence
Binding Sites
Cell Line, Transformed
Computer Graphics
DNA
Humans
Molecular Sequence Data
Promoter Regions, Genetic
Protein Conformation
Receptors, Retinoic Acid / genetics,  metabolism
Recombinant Fusion Proteins / metabolism
Saccharomyces cerevisiae
Transcription Factor TFIID
Transcription Factors / chemistry,  metabolism*
Transcription, Genetic*
Transcriptional Activation
Transfection
Tretinoin / pharmacology*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Adenovirus E1A Proteins; 0/Receptors, Retinoic Acid; 0/Recombinant Fusion Proteins; 0/Transcription Factor TFIID; 0/Transcription Factors; 302-79-4/Tretinoin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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