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Residue-specific 13C' CSA tensor principal components for ubiquitin: correlation between tensor components and hydrogen bonding.
MedLine Citation:
PMID:  17263416     Owner:  NLM     Status:  MEDLINE    
The residue-specific 13C' CSA tensor principal components, sigma(11), sigma(22), sigma(33), and the tensor orientation defined by the rotation angles beta and gamma have been determined by solution NMR for uniformly labeled ubiquitin partially aligned in four different media. Spurious chemical shift deviations due to solvent effects were corrected with an offset calculated by linear regression of the residual dipolar couplings and chemical shifts at increasing alignment strengths. Analysis of this effect revealed no obvious correlation to solvent exposure. Data obtained in solution from a protein offer a better sampling of 13C' CSA for different amino acid types in a complex heterogeneous environment, thereby allowing for the evaluation of structural variables that would be challenging to achieve by other methods. The 13C' CSA principal components cluster about the average values previously determined, and experimental correlations observed between sigma(11), sigma(22) tensorial components and C'O...H(N) hydrogen bonding are discussed. The inverse association of sigma(11) and sigma(22) exemplify the calculated and solid-state NMR observed effect on the tensor components by hydrogen bonding. We also show that 13C' CSA tensors are sensitive to hydrogen-bond length but not hydrogen-bond angle. This differentiation was previously unavailable. Similarly, hydrogen bonding to the conjugated NH of the same peptide plane has no detectable effect. Importantly, the observed weak correlations signify the presence of confounding influences such as nearest-neighbor effects, side-chain conformation, electrostatics, and other long-range factors to the 13C' CSA tensor. These analyses hold future potential for exploration provided that more accurate data from a larger number of proteins and alignments become available.
Robert A Burton; Nico Tjandra
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  129     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-31     Completed Date:  2007-03-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1321-6     Citation Subset:  IM    
Laboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, 50 South Drive, Bethesda, Maryland 20892, USA.
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MeSH Terms
Acrylamide / chemistry
Carbon Isotopes / chemistry
Hydrogen Bonding
Magnetic Resonance Spectroscopy
Peptides / chemistry
Polyethylene Glycols / chemistry
Protein Conformation
Solvents / chemistry
Ubiquitin / chemistry*
Reg. No./Substance:
0/Carbon Isotopes; 0/Peptides; 0/Polyethylene Glycols; 0/Solvents; 0/Ubiquitin; 79-06-1/Acrylamide

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