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Research on the steroidogenesis of proliferated Leydig cells in vitro.
MedLine Citation:
PMID:  23322215     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Several treatments for patients with primary hypogonadism are available, but these are associated with major complications. In this study, we explored the possibility of testosterone secretion by proliferated Leydig cells embedded in Matrigel with the aim of developing a source of endogenous testosterone supplement for recipients while reducing the need for donor material. Leydig cells were isolated and proliferated in vitro. The expression of 3β-hydroxysteroid dehydrogenase, cholesterol side-chain cleaving enzyme (CYP11A1), and 17α-hydroxylase/17,20-lyase (CYP17A1) was analyzed to confirm the purity and steroidogenesis capability of Leydig cells. The proliferated cells were then embedded in three-dimensional Matrigel, and following culture the supernatant medium was collected for measurement of testosterone concentration by radioimmunoassay. The biological behavior of the Leydig cells in the Matrigel was carefully observed under the microscope. Approximately 6.0 × 10(5) Leydig cells were obtained from one testis after primary culture in vitro. Aliquots of 1.0 × 10(5) Leydig cells were mixed with Matrigel, with the amount of cells in one pellet being equal to that in an adult testis. Leydig cells gradually formed aggregates when maintained in Matrigel. A rapid and constant linear increase in testosterone levels was detected in the supernatant medium. Our results demonstrate that Matrigel is a perfect support matrix for Leydig cells. Proliferated Leydig cells embedded in Matrigel have a great steroidogenesis reserve. In our study, they contributed to continuous steroidogenesis, which implies that the pellet may provide the physiological demand for endogenous androgen once engrafted in vivo. This system may ultimately provide a novel alternative treatment for people who are in need of androgen replacement.
Authors:
Liang Zhong; Jie Sun; Guo-Hua Liu; Ying-Jian Zhu; Jiang Zhu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-16
Journal Detail:
Title:  Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs     Volume:  -     ISSN:  1619-0904     ISO Abbreviation:  J Artif Organs     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815648     Medline TA:  J Artif Organs     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Urology, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine, No 100, Haining Road, Shanghai, 200080, People's Republic of China.
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