Document Detail


Research resource: RNA-Seq reveals unique features of the pancreatic β-cell transcriptome.
MedLine Citation:
PMID:  22915829     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The pancreatic β-cell is critical for the maintenance of glycemic control. Knowing the compendium of genes expressed in β-cells will further our understanding of this critical cell type and may allow the identification of future antidiabetes drug targets. Here, we report the use of next-generation sequencing to obtain nearly 1 billion reads from the polyadenylated RNA of islets and purified β-cells from mice. These data reveal novel examples of β-cell-specific splicing events, promoter usage, and over 1000 long intergenic noncoding RNA expressed in mouse β-cells. Many of these long intergenic noncoding RNA are β-cell specific, and we hypothesize that this large set of novel RNA may play important roles in β-cell function. Our data demonstrate unique features of the β-cell transcriptome.
Authors:
Gregory M Ku; Hail Kim; Ian W Vaughn; Matthew J Hangauer; Chang Myung Oh; Michael S German; Michael T McManus
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-08-21
Journal Detail:
Title:  Molecular endocrinology (Baltimore, Md.)     Volume:  26     ISSN:  1944-9917     ISO Abbreviation:  Mol. Endocrinol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-01     Completed Date:  2013-02-19     Revised Date:  2013-10-10    
Medline Journal Info:
Nlm Unique ID:  8801431     Medline TA:  Mol Endocrinol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1783-92     Citation Subset:  IM    
Affiliation:
513 Parnassus Avenue, Box 0534, San Francisco, California 94143-0534, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Female
Gene Expression Profiling
High-Throughput Nucleotide Sequencing*
Insulin-Secreting Cells / metabolism*
Male
Mice
Mice, Transgenic
Organ Specificity
Promoter Regions, Genetic
RNA Splicing
RNA, Long Untranslated / genetics,  metabolism
RNA, Messenger / genetics,  metabolism
Sequence Analysis, RNA
Transcriptome*
Tryptophan Hydroxylase / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
K08 DK087945/DK/NIDDK NIH HHS; K08DK087945/DK/NIDDK NIH HHS; P30DK063720/DK/NIDDK NIH HHS; R01DK021344/DK/NIDDK NIH HHS; RC1DK086290/DK/NIDDK NIH HHS; U01DK08954/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Long Untranslated; 0/RNA, Messenger; EC 1.14.16.4/Tph1 protein, mouse; EC 1.14.16.4/Tryptophan Hydroxylase
Comments/Corrections

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