| Research resource: RNA-Seq reveals unique features of the pancreatic β-cell transcriptome. | |
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MedLine Citation:
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PMID: 22915829 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The pancreatic β-cell is critical for the maintenance of glycemic control. Knowing the compendium of genes expressed in β-cells will further our understanding of this critical cell type and may allow the identification of future antidiabetes drug targets. Here, we report the use of next-generation sequencing to obtain nearly 1 billion reads from the polyadenylated RNA of islets and purified β-cells from mice. These data reveal novel examples of β-cell-specific splicing events, promoter usage, and over 1000 long intergenic noncoding RNA expressed in mouse β-cells. Many of these long intergenic noncoding RNA are β-cell specific, and we hypothesize that this large set of novel RNA may play important roles in β-cell function. Our data demonstrate unique features of the β-cell transcriptome. |
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Authors:
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Gregory M Ku; Hail Kim; Ian W Vaughn; Matthew J Hangauer; Chang Myung Oh; Michael S German; Michael T McManus |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-08-21 |
Journal Detail:
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Title: Molecular endocrinology (Baltimore, Md.) Volume: 26 ISSN: 1944-9917 ISO Abbreviation: Mol. Endocrinol. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-01 Completed Date: 2013-02-19 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 8801431 Medline TA: Mol Endocrinol Country: United States |
Other Details:
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Languages: eng Pagination: 1783-92 Citation Subset: IM |
Affiliation:
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513 Parnassus Avenue, Box 0534, San Francisco, California 94143-0534, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Female Gene Expression Profiling High-Throughput Nucleotide Sequencing* Insulin-Secreting Cells / metabolism* Male Mice Mice, Transgenic Organ Specificity Promoter Regions, Genetic RNA Splicing RNA, Long Untranslated / genetics, metabolism RNA, Messenger / genetics, metabolism Sequence Analysis, RNA Transcriptome* Tryptophan Hydroxylase / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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K08 DK087945/DK/NIDDK NIH HHS; K08DK087945/DK/NIDDK NIH HHS; P30DK063720/DK/NIDDK NIH HHS; R01DK021344/DK/NIDDK NIH HHS; RC1DK086290/DK/NIDDK NIH HHS; U01DK08954/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Long Untranslated; 0/RNA, Messenger; EC 1.14.16.4/Tph1 protein, mouse; EC 1.14.16.4/Tryptophan Hydroxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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