Document Detail

Rescue of shoot apical meristems of chrysanthemum by culturing on root tips.
MedLine Citation:
PMID:  14530865     Owner:  NLM     Status:  MEDLINE    
A new method to regenerate plants from leaf primordia-free shoot apical meristem domes (LP-free SAMs) was developed by establishing the meristem dome on the cut surface of root tips. Ten days after culture, the viable rate of LP-free SAMs of chrysanthemum 'Piato' attached to chrysanthemum root tips was >40%. Shoot regeneration was not observed from LP-free SAMs without the root tips. When LP-free SAMs of chrysanthemum were transferred to root tips of either petunia, cabbage, or carnation, the highest shoot regeneration rate was observed with cabbage root tips. Microscopic observation documented that the LP-free SAM temporarily adhered to the cut surface of the root tip of cabbage.
M Hosokawa; A Otake; Y Sugawara; T Hayashi; S Yazawa
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Publication Detail:
Type:  Journal Article     Date:  2003-10-03
Journal Detail:
Title:  Plant cell reports     Volume:  22     ISSN:  0721-7714     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-03-22     Completed Date:  2004-05-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  443-8     Citation Subset:  IM    
Laboratory of Vegetable and Ornamental Horticulture, Department of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan.
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MeSH Terms
Botany / methods
Chrysanthemum / growth & development*,  physiology
Meristem / growth & development
Plant Roots / growth & development

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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