| Requirements for unsaturated fatty acids for the induction on respiration in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 1104618 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Unsaturated fatty acids provided during the release from glucose repression were shown to be essential for derepression of respiration in an unsaturated fatty acid auxotroph of Saccharomyces cerevisiae (KD115). Cells derepressed in the presence of oleic acid contained three to six times as much cytochrome per cell as those derepressed in the absence of unsaturated fatty acid or those derepressed with eicosaenoic acid. The delta9 isomer was the most efficient of the cis-octadecenoic acid isomers in supporting that increase, and eicosaenoic acid supported an increase at only 15% the rate observed with oleic acid. Derepression, even in the presence of oleic acid, proceeded only after a lag of 3 hours. When glucose was removed prior to the addition of oleate, the lag was reduced by the time of the preincubation with glycerol. This result suggests that some processes necessary for increased respiration can proceed in the absence of an added unsaturated fatty acid, but these processes apparently require certain levels of unsaturated acids in the pre-existing lipids, since they occurred in cells whose membranes contained 50 mol % oleate, but not in cells containing only 20 mol %. These processes leading to eventual increased respiration were inhibited by cycloheximide but not chloramphenicol, suggesting that protein synthesis on cytoplasmic ribosomes but not mitochondrial ribosomes were required. Derepression in the absence of oleate for 3 hours lessened the inhibition or respiration induction by ethidium bromide. This result indicates that the transcription of mitochondrial DNA necessary for the induction of respiration may have occurred in the absence of added unsaturated fatty acid, but that some subsequent event required added esterified unsaturated fatty acid. |
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Authors:
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R W Walenga; W E Lands |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 250 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1975 Dec |
Date Detail:
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Created Date: 1976-02-09 Completed Date: 1976-02-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 9130-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chloramphenicol
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pharmacology Cycloheximide / pharmacology Cytochromes / metabolism Eicosanoic Acids / pharmacology Fatty Acids, Unsaturated / metabolism, pharmacology* Kinetics Oleic Acids / metabolism Oxygen Consumption / drug effects* Saccharomyces cerevisiae / drug effects, metabolism* Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
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0/Cytochromes; 0/Eicosanoic Acids; 0/Fatty Acids, Unsaturated; 0/Oleic Acids; 56-75-7/Chloramphenicol; 66-81-9/Cycloheximide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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