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Requirement for three signals in "T-independent" (lipopolysaccharide-induced) as well as in T-dependent B cell responses.
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MedLine Citation:
PMID:  6801177     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The requirements for different activation signals in the generation of plaque-forming cell (PFC) responses by positively selected B (surface immunoglobulin-positive) cells were analyzed in low-density cultures to minimize the possible effects of contaminating T cells. Using this system, it is demonstrated that not only in T helper cell (TH)-dependent but also in lipopolysaccharide (LPS)-dependent (i.e., so-called T-independent) PFC responses, the resting B cells have to receive at least three different signals: (a) a major histocompatibility complex (MHC)-specific TH signal that can be bypassed by LPS, (b) an antigen signal, and (c) a second TH signal medicated by MHC- and antigen-unspecific helper factor(s) for B cell responses (BHF) that cannot by bypassed by LPS. Specifically, contact of surface immunoglobulin-positive cells with cloned allo-I-A-specific TH or LPS induced a polyclonal PFC response without significant proliferation, whereas contact with BHF alone (obtained as supernatants from different cloned TH, EL-4 thymoma cells, or secondary mixed leukocyte culture cells) had no effect. Only when LPS, antigen, and BHF, or, alternatively, allo-TH (producing themselves BHF) and antigen were present did clonally expanded PFC responses occur. Thus, the data indicate that both an LPS (or specific TH) signal and an antigen signal are required to render the B cells responsive to BHF. BHF seems to act essentially as a nonspecific growth factor, whereas differentiation into antibody-secreting cells appears to be a preprogrammed consequence of B cell activation by an LPS or specific TH signal.
Authors:
R H Zubler; A L Glasebrook
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  155     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1982 Mar 
Date Detail:
Created Date:  1982-05-27     Completed Date:  1982-05-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  666-80     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibody-Producing Cells / immunology
B-Lymphocytes / immunology*
Clone Cells / metabolism
Erythrocytes / immunology
Hemolytic Plaque Technique
Humans
Interleukin-1
Kinetics
Lipopolysaccharides / pharmacology*
Lymphocyte Activation*
Mice
Mice, Inbred A
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred CBA
Mice, Inbred DBA
Proteins / pharmacology
Receptors, Antigen, B-Cell / immunology
Sheep
T-Lymphocytes / immunology*
Chemical
Reg. No./Substance:
0/Interleukin-1; 0/Lipopolysaccharides; 0/Proteins; 0/Receptors, Antigen, B-Cell
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 3 Year: 1982
Volume: 155 Issue: 3
First Page: 666 Last Page: 680
ID: 2186633
Publisher Id: 82144254
PubMed Id: 6801177

Requirement for three signals in "T-independent" (lipopolysaccharide- induced) as well as in T-dependent B cell responses


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