Document Detail


Requirement of successive protein syntheses for the progress of meiosis in Blepharisma.
MedLine Citation:
PMID:  6406249     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Germ nuclei of Blepharisma japonicum begin meiosis within a few hours when cells of complementary mating types conjugate. We synchronized the onset of conjugation and treated cells in different stages of meiosis with 10 micrograms/ml cycloheximide which strongly inhibits protein synthesis in this ciliate. Cycloheximide arrested meiosis at six stages: I, between pairing of cells and swelling of germ nuclei; II, leptotene; III, zygotene; IV, pachytene; XI, interkinesis; XII, prometaphase II. Five of these arrests were reversible. Puromycin (250-500 micrograms/ml) also inhibited the progress of meiosis, though to lesser extents. We propose that the progression of meiosis of B. japonicum requires at least six proteins which are synthesized sequentially during meiosis.
Authors:
E Friedl; A Miyake; K Heckmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  145     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1983 Apr 
Date Detail:
Created Date:  1983-07-15     Completed Date:  1983-07-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  105-13     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division
Ciliophora / cytology*,  metabolism
Cycloheximide / pharmacology
Protein Biosynthesis*
Puromycin / pharmacology
Chemical
Reg. No./Substance:
53-79-2/Puromycin; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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