Document Detail

Requirement for metalloendoprotease in exocytosis: evidence in mast cells and adrenal chromaffin cells.
MedLine Citation:
PMID:  2578889     Owner:  NLM     Status:  MEDLINE    
Exocytosis is initiated by the receptor-mediated influx of calcium that results in fusion of the secretory vesicle with the plasma membrane. We examined the possibility that calcium-dependent exocytosis in mast cells and adrenal chromaffin cells requires metalloendoprotease activity. Metalloendoprotease inhibitors and dipeptide substrates block exocytosis in these cells with the same specificity and dose dependency as that with which they interact with metalloendoproteases. Metalloendoprotease activity is identified in these cells with fluorogenic synthetic substrates, which also blocked exocytosis. Metalloendoprotease activity is highest in the plasma membrane of chromaffin cells. The metalloendoprotease appears to be required in exocytosis at a step dependent on or after calcium entry, since exocytosis initiated by direct calcium introduction in both mast cells and chromaffin cells is blocked by metalloendoprotease inhibitors.
D I Mundy; W J Strittmatter
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  40     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-04-24     Completed Date:  1985-04-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  645-56     Citation Subset:  IM    
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MeSH Terms
Adrenal Glands / cytology*
Calcimycin / pharmacology
Calcium / metabolism,  pharmacology
Cell Membrane / enzymology
Chromaffin System / cytology*
Concanavalin A / pharmacology
Dipeptides / pharmacology
Endopeptidases / physiology*
Exocytosis* / drug effects
Histamine Release / drug effects
Mast Cells / physiology*
Oligopeptides / metabolism
Phenanthrolines / pharmacology
Protease Inhibitors / pharmacology
Rats, Inbred Strains
Reg. No./Substance:
0/Dipeptides; 0/Oligopeptides; 0/Phenanthrolines; 0/Protease Inhibitors; 100307-95-7/2-aminobenzoylalanyl-glycyl-leucyl-alanyl-4-nitrobenzylamide; 11028-71-0/Concanavalin A; 52665-69-7/Calcimycin; 66-71-7/1,10-phenanthroline; 7440-70-2/Calcium; EC 3.4.-/Endopeptidases; EC 3.4.24.-/Metalloendopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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