Document Detail


Requirement for metalloendoprotease in exocytosis: evidence in mast cells and adrenal chromaffin cells.
MedLine Citation:
PMID:  2578889     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Exocytosis is initiated by the receptor-mediated influx of calcium that results in fusion of the secretory vesicle with the plasma membrane. We examined the possibility that calcium-dependent exocytosis in mast cells and adrenal chromaffin cells requires metalloendoprotease activity. Metalloendoprotease inhibitors and dipeptide substrates block exocytosis in these cells with the same specificity and dose dependency as that with which they interact with metalloendoproteases. Metalloendoprotease activity is identified in these cells with fluorogenic synthetic substrates, which also blocked exocytosis. Metalloendoprotease activity is highest in the plasma membrane of chromaffin cells. The metalloendoprotease appears to be required in exocytosis at a step dependent on or after calcium entry, since exocytosis initiated by direct calcium introduction in both mast cells and chromaffin cells is blocked by metalloendoprotease inhibitors.
Authors:
D I Mundy; W J Strittmatter
Related Documents :
618889 - Functional and morphological characterization of isolated bovine adrenal medullary cells.
18563439 - Ultrastructure of myoepithelial cells as a target cell in sialoadenitis of submandibula...
6131369 - Co-localization of vasoactive intestinal peptide (vip) and enkephalins in chromaffin ce...
24632839 - The toxoplasma acto-myoa motor complex is important but not essential for gliding motil...
12859019 - Influence of lithium on cell function in two different cell systems.
3305699 - Immunohistochemical localization of a thyroid hormone-binding protein (p55) in human ti...
3342439 - Structural changes produced in kupffer cells in the rat liver by injection of lipopolys...
20232349 - Characterization of uptake of folates by rat and human blood-brain barrier endothelial ...
2958329 - Lysosome response and cytoskeleton alteration in cell cultures exposed to airborne lead.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  40     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-04-24     Completed Date:  1985-04-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  645-56     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adrenal Glands / cytology*
Animals
Calcimycin / pharmacology
Calcium / metabolism,  pharmacology
Cattle
Cell Membrane / enzymology
Chromaffin System / cytology*
Concanavalin A / pharmacology
Dipeptides / pharmacology
Endopeptidases / physiology*
Exocytosis* / drug effects
Female
Histamine Release / drug effects
Mast Cells / physiology*
Metalloendopeptidases
Oligopeptides / metabolism
Phenanthrolines / pharmacology
Protease Inhibitors / pharmacology
Rats
Rats, Inbred Strains
Chemical
Reg. No./Substance:
0/Dipeptides; 0/Oligopeptides; 0/Phenanthrolines; 0/Protease Inhibitors; 100307-95-7/2-aminobenzoylalanyl-glycyl-leucyl-alanyl-4-nitrobenzylamide; 11028-71-0/Concanavalin A; 52665-69-7/Calcimycin; 66-71-7/1,10-phenanthroline; 7440-70-2/Calcium; EC 3.4.-/Endopeptidases; EC 3.4.24.-/Metalloendopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Stimulation of the T3-T cell receptor complex induces a membrane-potential-sensitive calcium influx.
Next Document:  Enzymatic cross-linking of involucrin and other proteins by keratinocyte particulates in vitro.