Document Detail

Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17.
MedLine Citation:
PMID:  17449009     Owner:  NLM     Status:  MEDLINE    
The operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodococcus sp. strain DK17. RT-PCR experiments using gene-specific primers showed that both the pDK2- and the pDK3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. The doubling time of the pDK2-cured mutant strain DK176 in minimal liquid medium with 5mM phthalate is 52.5% of that of the wild-type strain DK17. The data indicate that both copies of the phthalate operon are equally functional in DK17, and gene dosage is the main reason for slower growth of DK176 on phthalate.
Ki Young Choi; Dockyu Kim; Jong-Chan Chae; Gerben J Zylstra; Eungbin Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-04-10
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  357     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-02     Completed Date:  2007-07-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  766-71     Citation Subset:  IM    
Department of Biology, Yonsei University, Seoul 120-749, Republic of Korea.
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MeSH Terms
Carboxy-Lyases / genetics,  metabolism
Cell Division / drug effects,  genetics,  physiology
Gene Duplication*
Gene Expression Regulation, Bacterial / drug effects
Gene Order
Genes, Bacterial
Metabolic Networks and Pathways
Operon / genetics*
Phthalic Acids / metabolism*,  pharmacology
Plasmids / genetics
Rhodococcus / drug effects,  genetics,  metabolism*
Reg. No./Substance:
0/Phthalic Acids; 88-99-3/phthalic acid; EC 4.1.1.-/Carboxy-Lyases; EC,5-dihydroxyphthalate decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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