Document Detail


Requirement of cell division for muscle actin expression in the primary muscle cell lineage of ascidian embryos.
MedLine Citation:
PMID:  2806119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of cell division in the expression of muscle actin and its relationship to acetylcholinesterase (AChE) development was examined in cleavage-arrested embryos of the ascidian Styela. Muscle actin expression was detected by two-dimensional gel electrophoresis of radioactively labelled proteins and by in situ hybridization with a cDNA probe, whereas AChE activity was assayed by enzyme histochemistry. In the majority of cases, muscle actin expression was first detected in embryos arrested after the 16-cell stage. Some embryos showed muscle actin expression after arrest at the 8-cell stage, however, muscle actin mRNA did not accumulate in embryos arrested at earlier cleavages. The cells that expressed muscle actin in 8- to 64-cell cleavage-arrested embryos belonged to the primary muscle lineage; secondary muscle cell precursors did not express muscle actin. Zygotic muscle actin mRNA appeared to accumulate with myoplasmic pigment granules in the perinuclear region of cleavage-arrested embryos, suggesting that the myoplasm may have a role in the organization of muscle cells. In contrast to muscle actin, AChE was detected in a small proportion of embryos treated with cytochalasin as early as the 1- or 2-cell stage, and most embryos treated with cytochalasin at later cleavages expressed this enzyme in some of their cells. Most primary muscle lineage cells expressed both muscle actin mRNA and AChE, however, some cells expressed only muscle actin mRNA or AChE. The results suggest that at least three cleavages are required for muscle actin expression and that muscle actin and AChE expression can be uncoupled in cleavage-arrested embryos.
Authors:
W R Jeffery
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  105     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-12-06     Completed Date:  1989-12-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  75-84     Citation Subset:  IM    
Affiliation:
Department of Zoology, University of Texas, Austin 78712.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism*
Animals
Cell Division*
Muscles / metabolism*
Urochordata / growth & development*
Grant Support
ID/Acronym/Agency:
HD-13970/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Actins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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