Document Detail


Requirement of c-jun transcription factor on the mouse mast cell protease-6 expression in the mast cells.
MedLine Citation:
PMID:  15464728     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mast cell tryptases may be a key mediator in mast cell-mediated inflammatory reactions, and these expressions can be regulated by microenvironmental factors of tissues, particularly stem cell factor. In the present study, we investigated whether the transcription of mouse mast cell protease-6 (mMCP-6) gene was caused by SCF-mediated c-jun. We observed that mMCP-6 mRNA was expressed by overexpression of c-jun in the immature mast cell line in which both mMCP-6 and c-kit receptor are negative. The c-jun increased synergistically the luciferase activity of mMCP-6 promoter through the direct interaction with mi transcription factor (MITF). The synergic effect of c-jun with MITF was abolished by deletion of sequence between nt -171 and -151 in the mMCP-6 promoter. Furthermore, the level of mMCP-6 mRNA in mast cells was attenuated by the introduction of dominant negative c-jun (TAM-67) and the treatment of Jun N-terminal kinase inhibitor, SP600125. These results show that c-jun might play a role in regulating the transcription of mMCP-6 gene in mast cells stimulated by SCF.
Authors:
Dae-Ki Kim; Young-Mi Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  431     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-10-06     Completed Date:  2004-12-02     Revised Date:  2010-10-01    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  71-8     Citation Subset:  IM    
Affiliation:
Department of Immunology, Chonbuk National University Medical School, Jeonbuk, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
DNA-Binding Proteins / metabolism
Gene Expression Regulation / physiology*
Mast Cells / enzymology*,  metabolism
Mice
Microphthalmia-Associated Transcription Factor
Molecular Sequence Data
Peptide Fragments / metabolism
Proto-Oncogene Proteins c-jun / metabolism*
Proto-Oncogene Proteins c-kit / genetics,  metabolism
RNA, Messenger / metabolism
Serine Endopeptidases / biosynthesis,  genetics*
Stem Cell Factor / metabolism
Transcription Factors / metabolism
Transfection
Tryptases
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Mcpt6 protein, mouse; 0/Microphthalmia-Associated Transcription Factor; 0/Mitf protein, mouse; 0/Peptide Fragments; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 0/Stem Cell Factor; 0/TAM67 peptide; 0/Transcription Factors; EC 2.7.10.1/Proto-Oncogene Proteins c-kit; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.59/Mcpt6 protein, rat; EC 3.4.21.59/TPSB1 protein, rat; EC 3.4.21.59/Tpsab1 protein, mouse; EC 3.4.21.59/Tryptases

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