Document Detail

Requirement of NMB0065 for connecting assembly and export of sialic acid capsular polysaccharides in Neisseria meningitidis.
MedLine Citation:
PMID:  20215001     Owner:  NLM     Status:  MEDLINE    
Capsule expression in Neisseria meningitidis is encoded by the cps locus comprised of genes required for biosynthesis and surface translocation. Located adjacent to the gene encoding the polysialyltransferase in serogroups expressing sialic acid-containing capsule, NMB0065 is likely a member of the cps locus, but it is not found in serogroups A or X that express non-sialic acid capsules. To further understand its role in CPS expression, NMB0065 mutants were created in the serogroups B, C and Y strains. The mutants were as sensitive as unencapsulated strains to killing by normal human serum, despite producing near wild-type levels of CPS. Absence of surface expression of capsule was suggested by increased surface hydrophobicity and confirmed by immunogold electron microscopy, which revealed the presence of large vacuoles containing CPS within the cell. GC-MS and NMR analyses of purified capsule from the mutant revealed no apparent changes in polymer structures and lipid anchors. Mutants of NMB0065 homologues in other sialic acid CPS expressing meningococcal serogroups had similar phenotypes. Thus, NMB0065 (CtrG) is not involved in biosynthesis or lipidation of sialic acid-containing capsule but encodes a protein required for proper coupling of the assembly complex to the membrane transport complex allowing surface expression of CPS.
Rhonda I Hobb; Yih-Ling Tzeng; Biswa P Choudhury; Russell W Carlson; David S Stephens
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-03-07
Journal Detail:
Title:  Microbes and infection / Institut Pasteur     Volume:  12     ISSN:  1769-714X     ISO Abbreviation:  Microbes Infect.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-10     Completed Date:  2010-09-21     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  100883508     Medline TA:  Microbes Infect     Country:  France    
Other Details:
Languages:  eng     Pagination:  476-87     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Masson SAS. All rights reserved.
Department of Medicine, Emory University School of Medicine, Atlanta, GA, United States.
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MeSH Terms
Amino Acid Sequence
Bacterial Capsules / metabolism*
Blood Bactericidal Activity
Cloning, Molecular
Genome, Bacterial
Membrane Transport Proteins / metabolism
Microscopy, Electron
Molecular Sequence Data
N-Acetylneuraminic Acid / metabolism*
Neisseria meningitidis / genetics*,  metabolism*,  ultrastructure
Sequence Alignment
Grant Support
AI061031/AI/NIAID NIH HHS; AI40247/AI/NIAID NIH HHS; R01 AI040247/AI/NIAID NIH HHS; R01 AI040247-03/AI/NIAID NIH HHS; R01 AI040247-04/AI/NIAID NIH HHS; R01 AI040247-05A1/AI/NIAID NIH HHS; R01 AI040247-06/AI/NIAID NIH HHS; R01 AI040247-07/AI/NIAID NIH HHS; R01 AI040247-08/AI/NIAID NIH HHS; R01 AI040247-09/AI/NIAID NIH HHS; R01 AI061031-01A1/AI/NIAID NIH HHS; R01 AI061031-02/AI/NIAID NIH HHS; R01 AI061031-03/AI/NIAID NIH HHS; R01 AI061031-04/AI/NIAID NIH HHS; R01 AI061031-05/AI/NIAID NIH HHS
Reg. No./Substance:
0/Membrane Transport Proteins; 131-48-6/N-Acetylneuraminic Acid

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