Document Detail


Requirement of CHROMOMETHYLASE3 for maintenance of CpXpG methylation.
MedLine Citation:
PMID:  11349138     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Epigenetic silenced alleles of the Arabidopsis SUPERMAN locus (the clark kent alleles) are associated with dense hypermethylation at noncanonical cytosines (CpXpG and asymmetric sites, where X = A, T, C, or G). A genetic screen for suppressors of a hypermethylated clark kent mutant identified nine loss-of-function alleles of CHROMOMETHYLASE3 (CMT3), a novel cytosine methyltransferase homolog. These cmt3 mutants display a wild-type morphology but exhibit decreased CpXpG methylation of the SUP gene and of other sequences throughout the genome. They also show reactivated expression of endogenous retrotransposon sequences. These results show that a non-CpG DNA methyltransferase is responsible for maintaining epigenetic gene silencing.
Authors:
A M Lindroth; X Cao; J P Jackson; D Zilberman; C M McCallum; S Henikoff; S E Jacobsen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2001-05-10
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  292     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-06-15     Completed Date:  2001-07-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2077-80     Citation Subset:  IM    
Affiliation:
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF364174
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MeSH Terms
Descriptor/Qualifier:
Alleles
Amino Acid Sequence
Arabidopsis / genetics*,  metabolism
Arabidopsis Proteins*
Base Sequence
Chromosome Mapping
Cloning, Molecular
CpG Islands
Crosses, Genetic
Cytosine / metabolism
DNA Methylation*
DNA-Cytosine Methylases / chemistry,  genetics*,  metabolism*
Dinucleoside Phosphates / metabolism
Gene Expression Regulation, Plant
Gene Silencing*
Genes, Plant
Molecular Sequence Data
Mutagenesis
Oligonucleotides / metabolism*
Phenotype
Protein Structure, Tertiary
Retroelements
Transcription Factors / genetics*
Grant Support
ID/Acronym/Agency:
GM07104/GM/NIGMS NIH HHS; GM07185/GM/NIGMS NIH HHS; GM29009/GM/NIGMS NIH HHS; GM60398/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Dinucleoside Phosphates; 0/Oligonucleotides; 0/Retroelements; 0/Transcription Factors; 0/superman protein, Arabidopsis; 2382-65-2/cytidylyl-3'-5'-guanosine; 71-30-7/Cytosine; EC 2.1.1.-/DNA-Cytosine Methylases; EC 2.1.1.37/CMT3 protein, Arabidopsis

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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