| A requirement for bid for induction of apoptosis by photodynamic therapy with a lysosome- but not a mitochondrion-targeted photosensitizer. | |
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MedLine Citation:
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PMID: 20553412 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Photodynamic therapy (PDT) with lysosome-targeted photosensitizers induces the intrinsic pathway of apoptosis via the cleavage and activation of the BH3-only protein Bid by proteolytic enzymes released from photodisrupted lysosomes. To investigate the role of Bid in apoptosis induction and the role of damaged lysosomes on cell killing by lysosome-targeted PDT, we compared the responses of wild type and Bid-knock-out murine embryonic fibroblasts toward a mitochondrion/endoplasmic reticulum-binding photosensitizer, Pc 4, and a lysosome-targeted sensitizer, Pc 181. Whereas apoptosis and overall cell killing were induced equally well by Pc 4-PDT in both cell lines, Bid(-/-) cells were relatively resistant to induction of apoptosis and to overall killing following PDT with Pc 181, particularly at low PDT doses. Thus, Bid is critical for the induction of apoptosis caused by PDT with the lysosome-specific sensitizers, but dispensable for PDT targeted to other membranes. |
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Authors:
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Song-Mao Chiu; Liang-Yan Xue; Minh Lam; Myriam E Rodriguez; Ping Zhang; Malcolm E Kenney; Anna-Liisa Nieminen; Nancy L Oleinick |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Photochemistry and photobiology Volume: 86 ISSN: 1751-1097 ISO Abbreviation: Photochem. Photobiol. Publication Date: 2010 Sep-Oct |
Date Detail:
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Created Date: 2010-11-05 Completed Date: 2011-03-31 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 0376425 Medline TA: Photochem Photobiol Country: United States |
Other Details:
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Languages: eng Pagination: 1161-73 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Journal Compilation. The American Society of Photobiology. |
Affiliation:
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Department of Radiation Oncology, Case Western Reserve University, Cleveland, OH, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects BH3 Interacting Domain Death Agonist Protein / genetics, physiology* Blotting, Western Cell Survival / drug effects Dose-Response Relationship, Drug Drug Delivery Systems* Gene Knockout Techniques Mitochondria / drug effects Molecular Structure Muramidase / drug effects, radiation effects* Photochemotherapy* Photosensitizing Agents / radiation effects |
| Grant Support | |
ID/Acronym/Agency:
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P01 CA048735-12/CA/NCI NIH HHS; P01 CA48735/CA/NCI NIH HHS; R01 CA083917-07/CA/NCI NIH HHS; R01 CA119079/CA/NCI NIH HHS; R01 CA119079-04/CA/NCI NIH HHS; R01 CA83917/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/BH3 Interacting Domain Death Agonist Protein; 0/BID protein, human; 0/Photosensitizing Agents; EC 3.2.1.17/Muramidase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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