Document Detail

Requirement for acetyl-CoA carboxylase in Trypanosoma brucei is dependent upon the growth environment.
MedLine Citation:
PMID:  21306439     Owner:  NLM     Status:  MEDLINE    
Trypanosoma brucei, the causative agent of human African trypanosomiasis, possesses two fatty acid synthesis pathways: a major de novo synthesis pathway in the ER and a mitochondrial pathway. The 2-carbon donor for both pathways is malonyl-CoA, which is synthesized from acetyl-CoA by Acetyl-CoA carboxylase (ACC). Here, we show that T. brucei ACC shares the same enzyme architecture and moderate ∼ 30% identity with yeast and human ACCs. ACC is cytoplasmic and appears to be distributed throughout the cell in numerous puncta distinct from glycosomes and other organelles. ACC is active in both bloodstream and procyclic forms. Reduction of ACC activity by RNA interference (RNAi) resulted in a stage-specific phenotype. In procyclic forms, ACC RNAi resulted in 50-75% reduction in fatty acid elongation and a 64% reduction in growth in low-lipid media. In bloodstream forms, ACC RNAi resulted in a minor 15% decrease in fatty acid elongation and no growth defect in culture, even in low-lipid media. However, ACC RNAi did attenuate virulence in a mouse model of infection. Thus the requirement for ACC in T. brucei is dependent upon the growth environment in two different life cycle stages.
Patrick A Vigueira; Kimberly S Paul
Related Documents :
17699519 - Identification of residues important for agonist recognition and activation in gpr40.
8033289 - Oxidation of furan fatty acids by soybean lipoxygenase-1 in the presence of linoleic acid.
1134219 - Oxidative desaturation of alpha-linoleic, linoleic, and stearic acids by human liver mi...
18515829 - The utilization and desaturation of oleate and linoleate during glycerolipid biosynthes...
11756069 - Unsaturated fatty acids selectively induce an inflammatory environment in human endothe...
19571159 - Hepatomegaly induced by trans-10,cis-12 conjugated linoleic acid in adult hamsters fed ...
10811809 - A novel system a isoform mediating na+/neutral amino acid cotransport.
6124929 - Electrophysiological analysis of rat renal sugar and amino acid transport. v. acidic am...
17943179 - Metabolism and biological activities of topical 4-oxoretinoids in mouse skin.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-02-16
Journal Detail:
Title:  Molecular microbiology     Volume:  80     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-25     Completed Date:  2011-07-15     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  117-32     Citation Subset:  IM    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acetyl-CoA Carboxylase / genetics,  metabolism*
Blotting, Northern
RNA Interference
Signal Transduction / genetics,  physiology
Trypanosoma brucei brucei / enzymology*,  genetics,  growth & development*
Grant Support
Reg. No./Substance:
EC Carboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Predictors of symptomatic lymphocele after radical prostatectomy and bilateral pelvic lymph node dis...
Next Document:  In Salmonella enterica, the sirtuin-dependent protein acylation/deacylation system (SDPADS) maintain...