Document Detail


Reptin52 expression during in vitro neural differentiation of human embryonic stem cells.
MedLine Citation:
PMID:  19444951     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human embryonic stem cells (hESCs) give rise to all somatic cell types, including neural cells such as astrocytes, oligodendrocytes and neurons. Commitment of hESC to a neural fate can be achieved via selection and expansion of developing neural stem cells, which, grown into non-adhering colonies called neurospheres, express nestin, a neurofilament marker. Analysis of hESC and hESC-derived neural stem cell nuclear extracts revealed an increased expression of Reptin52 in neurosphere nuclei. The increase in Reptin52 was evident throughout directed neuronal differentiation as assessed by western blotting, quantitative RT-PCR and immunocytochemistry. Reptin52 serves a pivotal regulatory role in nuclear activities such as transcription regulation and histone modification. In that regard, co-immunoprecipitation experiments showed that binding partners of Reptin52 (Pontin52, beta-catenin and ATF-2) associate with this regulatory protein in hESC-derived neuronal precursors. Moreover, expression of two of these proteins (beta-catenin - the end product of the Wnt signaling pathway - and ATF-2) is coordinately regulated with Reptin52.
Authors:
Miguel Barthéléry; Amritha Jaishankar; Ugur Salli; Kent E Vrana
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neuroscience letters     Volume:  452     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-05-13     Completed Date:  2009-06-11     Revised Date:  2010-02-04    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  47-51     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Penn State University College of Medicine, Hershey, PA 17033, United States.
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MeSH Terms
Descriptor/Qualifier:
Activating Transcription Factors / metabolism
Antibodies / pharmacology
Blood Proteins / metabolism
Carrier Proteins / genetics,  immunology,  metabolism*
Cell Differentiation / drug effects,  physiology*
Cell Line
DNA Helicases / genetics,  immunology,  metabolism*
Embryonic Stem Cells / physiology*
Humans
Immunoprecipitation
Intermediate Filament Proteins / genetics,  metabolism
Nerve Tissue Proteins / genetics,  metabolism
Neurons / drug effects,  metabolism*
RNA, Messenger / metabolism
Time Factors
beta Catenin / metabolism
Chemical
Reg. No./Substance:
0/Activating Transcription Factors; 0/Antibodies; 0/Blood Proteins; 0/Carrier Proteins; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/RUVBL1 protein, human; 0/RUVBL2 protein, human; 0/beta Catenin; 0/nestin; EC 3.6.1.-/DNA Helicases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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