Document Detail


Reptilian melanomacrophages function under conditions of hypothermia: observations on phagocytic behavior.
MedLine Citation:
PMID:  10614577     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Melanomacrophages (MMs) were removed from livers of turtles of three North American families and cultured. J774 mammalian macrophages were similarly cultured and the MMs were exposed to E. coli(fluor) at approximately 2, 7, 27, and 37 degrees C. At least one third of the MMs continued to function at the low temperatures where less than 2% of the mammalian cells incorporated bacteria deeply into the cytoplasm. In most instances, when the bacteria were not internalized deeply into the cytoplasm, they became stationary just inside, or within, the cell membrane. The MMs were significantly less efficient than the mammalian cells at 37 degrees C and significantly more efficient at 2 and 7 degrees C. In general, it appears that MMs are never as efficient as mammalian macrophages under the most ideal temperatures for the cell but they are capable of functioning at reasonable levels at temperature extremes. The observations are suggestive of a genetic mechanism functioning in the MMs that is rarely expressed in J774 cells under conditions of hypothermia. MMs in vitro and probably in vivo consume bacteria, fungi, attach to helminth eggs, and consume old erythrocytes.
Authors:
J C Johnson; T Schwiesow; A K Ekwall; J L Christiansen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society     Volume:  12     ISSN:  0893-5785     ISO Abbreviation:  Pigment Cell Res.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-01-27     Completed Date:  2000-01-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800247     Medline TA:  Pigment Cell Res     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  376-82     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Osteopathic Medicine and Health Sciences, Des Moines, Iowa 50312, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Helminths
Hemosiderin / analysis
Hibernation
Hypothermia / physiopathology*
Liver / cytology
Macrophages / physiology*
Melanins / physiology*
Phagocytosis*
Temperature
Turtles / immunology,  physiology*
Chemical
Reg. No./Substance:
0/Melanins; 9011-92-1/Hemosiderin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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