Document Detail


Reprogramming within hours following nuclear transfer into mouse but not human zygotes.
MedLine Citation:
PMID:  21971503     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fertilized mouse zygotes can reprogram somatic cells to a pluripotent state. Human zygotes might therefore be useful for producing patient-derived pluripotent stem cells. However, logistical, legal and social considerations have limited the availability of human eggs for research. Here we show that a significant number of normal fertilized eggs (zygotes) can be obtained for reprogramming studies. Using these zygotes, we found that when the zygotic genome was replaced with that of a somatic cell, development progressed normally throughout the cleavage stages, but then arrested before the morula stage. This arrest was associated with a failure to activate transcription in the transferred somatic genome. In contrast to human zygotes, mouse zygotes reprogrammed the somatic cell genome to a pluripotent state within hours after transfer. Our results suggest that there may be a previously unappreciated barrier to successful human nuclear transfer, and that future studies could focus on the requirements for genome activation.
Authors:
Dieter Egli; Alice E Chen; Genevieve Saphier; Justin Ichida; Claire Fitzgerald; Kathryn J Go; Nicole Acevedo; Jay Patel; Manfred Baetscher; William G Kearns; Robin Goland; Rudolph L Leibel; Douglas A Melton; Kevin Eggan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-10-04
Journal Detail:
Title:  Nature communications     Volume:  2     ISSN:  2041-1723     ISO Abbreviation:  Nat Commun     Publication Date:  2011  
Date Detail:
Created Date:  2011-10-05     Completed Date:  2012-01-18     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  101528555     Medline TA:  Nat Commun     Country:  England    
Other Details:
Languages:  eng     Pagination:  488     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GEO/GSE24631;  GSE28811
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MeSH Terms
Descriptor/Qualifier:
Animals
Humans
Mice
Mitosis
Nuclear Transfer Techniques*
Zygote / cytology*
Grant Support
ID/Acronym/Agency:
K99 NS077435/NS/NINDS NIH HHS; R01 HD045732-03/HD/NICHD NIH HHS; R01 HD046732/HD/NICHD NIH HHS; R01 HD046732-08/HD/NICHD NIH HHS
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