| Reprogramming within hours following nuclear transfer into mouse but not human zygotes. | |
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MedLine Citation:
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PMID: 21971503 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fertilized mouse zygotes can reprogram somatic cells to a pluripotent state. Human zygotes might therefore be useful for producing patient-derived pluripotent stem cells. However, logistical, legal and social considerations have limited the availability of human eggs for research. Here we show that a significant number of normal fertilized eggs (zygotes) can be obtained for reprogramming studies. Using these zygotes, we found that when the zygotic genome was replaced with that of a somatic cell, development progressed normally throughout the cleavage stages, but then arrested before the morula stage. This arrest was associated with a failure to activate transcription in the transferred somatic genome. In contrast to human zygotes, mouse zygotes reprogrammed the somatic cell genome to a pluripotent state within hours after transfer. Our results suggest that there may be a previously unappreciated barrier to successful human nuclear transfer, and that future studies could focus on the requirements for genome activation. |
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Authors:
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Dieter Egli; Alice E Chen; Genevieve Saphier; Justin Ichida; Claire Fitzgerald; Kathryn J Go; Nicole Acevedo; Jay Patel; Manfred Baetscher; William G Kearns; Robin Goland; Rudolph L Leibel; Douglas A Melton; Kevin Eggan |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-10-04 |
Journal Detail:
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Title: Nature communications Volume: 2 ISSN: 2041-1723 ISO Abbreviation: Nat Commun Publication Date: 2011 |
Date Detail:
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Created Date: 2011-10-05 Completed Date: 2012-01-18 Revised Date: 2012-10-09 |
Medline Journal Info:
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Nlm Unique ID: 101528555 Medline TA: Nat Commun Country: England |
Other Details:
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Languages: eng Pagination: 488 Citation Subset: IM |
Affiliation:
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Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts 02138, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GEO/GSE24631; GSE28811 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Humans Mice Mitosis Nuclear Transfer Techniques* Zygote / cytology* |
| Grant Support | |
ID/Acronym/Agency:
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K99 NS077435/NS/NINDS NIH HHS; R01 HD045732-03/HD/NICHD NIH HHS; R01 HD046732-08/HD/NICHD NIH HHS |
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