| Reprogramming of somatic cells induced by fusion of embryonic stem cells using hemagglutinating virus of Japan envelope (HVJ-E). | |
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MedLine Citation:
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PMID: 20338150 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this research, hemagglutinating virus of Japan envelope (HVJ-E) was used to reprogram somatic cells by fusion with mouse embryonic stem (ES) cells. Neomycin-resistant mouse embryonic fibroblasts (MEFs) were used as somatic cells. Nanog-overexpressing puromycin-resistant EB3 cells were used as mouse ES cells. These two cells were fused by exposing to HVJ-E and the generated fusion cells were selected by puromycin and G418 to get the stable fusion cell line. The fusion cells form colonies in feeder-free culture system. Microsatellite analysis of the fusion cells showed that they possessed genes from both ES cells and fibroblasts. The fusion cells were tetraploid, had alkali phosphatase activity, and expressed stem cell marker genes such as Pou5f1, Nanog, and Sox2, but not the fibroblast cell marker genes such as Col1a1 and Col1a2. The pluripotency of fusion cells was confirmed by their expression of marker genes for all the three germ layers after differentiation induction, and by their ability to form teratoma which contained all the three primary layers. Our results show that HVJ-E can be used as a fusion reagent for reprogramming of somatic cells. |
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Authors:
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Xiao-shan Yue; Masako Fujishiro; Masashi Toyoda; Toshihiro Akaike; Yoshihiro Ito |
Publication Detail:
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Type: Journal Article Date: 2010-03-23 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 394 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-19 Completed Date: 2010-05-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 1053-7 Citation Subset: IM |
Copyright Information:
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Crown Copyright 2010. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Fusion / methods* Embryonic Stem Cells / cytology, physiology* Fibroblasts / cytology, physiology* Genetic Markers Karyotyping Mice Microsatellite Repeats Nuclear Reprogramming* Pluripotent Stem Cells / cytology, physiology* Sendai virus* |
| Chemical | |
Reg. No./Substance:
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0/Genetic Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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