Document Detail


Reprogramming of somatic cells induced by fusion of embryonic stem cells using hemagglutinating virus of Japan envelope (HVJ-E).
MedLine Citation:
PMID:  20338150     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this research, hemagglutinating virus of Japan envelope (HVJ-E) was used to reprogram somatic cells by fusion with mouse embryonic stem (ES) cells. Neomycin-resistant mouse embryonic fibroblasts (MEFs) were used as somatic cells. Nanog-overexpressing puromycin-resistant EB3 cells were used as mouse ES cells. These two cells were fused by exposing to HVJ-E and the generated fusion cells were selected by puromycin and G418 to get the stable fusion cell line. The fusion cells form colonies in feeder-free culture system. Microsatellite analysis of the fusion cells showed that they possessed genes from both ES cells and fibroblasts. The fusion cells were tetraploid, had alkali phosphatase activity, and expressed stem cell marker genes such as Pou5f1, Nanog, and Sox2, but not the fibroblast cell marker genes such as Col1a1 and Col1a2. The pluripotency of fusion cells was confirmed by their expression of marker genes for all the three germ layers after differentiation induction, and by their ability to form teratoma which contained all the three primary layers. Our results show that HVJ-E can be used as a fusion reagent for reprogramming of somatic cells.
Authors:
Xiao-shan Yue; Masako Fujishiro; Masashi Toyoda; Toshihiro Akaike; Yoshihiro Ito
Publication Detail:
Type:  Journal Article     Date:  2010-03-23
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  394     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-19     Completed Date:  2010-05-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1053-7     Citation Subset:  IM    
Copyright Information:
Crown Copyright 2010. Published by Elsevier Inc. All rights reserved.
Affiliation:
Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fusion / methods*
Embryonic Stem Cells / cytology,  physiology*
Fibroblasts / cytology,  physiology*
Genetic Markers
Karyotyping
Mice
Microsatellite Repeats
Nuclear Reprogramming*
Pluripotent Stem Cells / cytology,  physiology*
Sendai virus*
Chemical
Reg. No./Substance:
0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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