Document Detail


Reprogramming of somatic cells after fusion with induced pluripotent stem cells and nuclear transfer embryonic stem cells.
MedLine Citation:
PMID:  19637940     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study we examine whether a somatic cell, once returned to a pluripotent state, gains the ability to reprogram other somatic cells. We reprogrammed mouse embryonic fibroblasts by viral induction of oct4, sox2, c-myc, and klf-4 genes. Upon fusion of the resulting iPS cells with somatic cells harboring an Oct4-GFP transgene we observed, GFP expression along with activation of Oct4 from the somatic genome, expression of key pluripotency genes, and positive immunostaining for Oct4, SSEA-1, and alkaline phosphatase. The iPS-somatic hybrids had the ability to differentiate into cell types indicative of the three germ layers and were able to localize to the inner cell mass of aggregated embryos. Furthermore, ntES cells were used as fusion partners to generate hybrids, which were also confirmed to be reprogrammed to a pluripotent state. These results demonstrate that once a somatic cell nucleus is reprogrammed, it acquires the capacity and potency to reprogram other somatic cells by cell fusion and shares this functional property with normal embryonic stem (ES) cells.
Authors:
Huseyin Sumer; Karen L Jones; Jun Liu; Corey Heffernan; Pollyanna A Tat; Kyle R Upton; Paul J Verma
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stem cells and development     Volume:  19     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-26     Completed Date:  2010-06-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  239-46     Citation Subset:  IM    
Affiliation:
Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia. huseyin.sumer@med.monash.edu.au
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fusion
Cell Transplantation / methods
Cells, Cultured
Embryo, Mammalian / cytology
Embryonic Stem Cells / cytology*,  metabolism
Female
Fibroblasts / cytology*,  metabolism
Green Fluorescent Proteins / genetics,  metabolism
Hybrid Cells / cytology*,  metabolism
Immunohistochemistry
Induced Pluripotent Stem Cells / cytology*,  metabolism,  transplantation
Male
Mice
Mice, SCID
Mice, Transgenic
Nuclear Reprogramming
Octamer Transcription Factor-3 / genetics,  metabolism
Pregnancy
Reverse Transcriptase Polymerase Chain Reaction
Teratoma / genetics,  metabolism,  pathology
Transduction, Genetic
Chemical
Reg. No./Substance:
0/Octamer Transcription Factor-3; 0/Pou5f1 protein, mouse; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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