| Reprogramming of somatic cells after fusion with induced pluripotent stem cells and nuclear transfer embryonic stem cells. | |
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MedLine Citation:
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PMID: 19637940 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study we examine whether a somatic cell, once returned to a pluripotent state, gains the ability to reprogram other somatic cells. We reprogrammed mouse embryonic fibroblasts by viral induction of oct4, sox2, c-myc, and klf-4 genes. Upon fusion of the resulting iPS cells with somatic cells harboring an Oct4-GFP transgene we observed, GFP expression along with activation of Oct4 from the somatic genome, expression of key pluripotency genes, and positive immunostaining for Oct4, SSEA-1, and alkaline phosphatase. The iPS-somatic hybrids had the ability to differentiate into cell types indicative of the three germ layers and were able to localize to the inner cell mass of aggregated embryos. Furthermore, ntES cells were used as fusion partners to generate hybrids, which were also confirmed to be reprogrammed to a pluripotent state. These results demonstrate that once a somatic cell nucleus is reprogrammed, it acquires the capacity and potency to reprogram other somatic cells by cell fusion and shares this functional property with normal embryonic stem (ES) cells. |
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Authors:
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Huseyin Sumer; Karen L Jones; Jun Liu; Corey Heffernan; Pollyanna A Tat; Kyle R Upton; Paul J Verma |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Stem cells and development Volume: 19 ISSN: 1557-8534 ISO Abbreviation: Stem Cells Dev. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-26 Completed Date: 2010-06-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101197107 Medline TA: Stem Cells Dev Country: United States |
Other Details:
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Languages: eng Pagination: 239-46 Citation Subset: IM |
Affiliation:
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Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia. huseyin.sumer@med.monash.edu.au |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Fusion Cell Transplantation / methods Cells, Cultured Embryo, Mammalian / cytology Embryonic Stem Cells / cytology*, metabolism Female Fibroblasts / cytology*, metabolism Green Fluorescent Proteins / genetics, metabolism Hybrid Cells / cytology*, metabolism Immunohistochemistry Induced Pluripotent Stem Cells / cytology*, metabolism, transplantation Male Mice Mice, SCID Mice, Transgenic Nuclear Reprogramming Octamer Transcription Factor-3 / genetics, metabolism Pregnancy Reverse Transcriptase Polymerase Chain Reaction Teratoma / genetics, metabolism, pathology Transduction, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Octamer Transcription Factor-3; 0/Pou5f1 protein, mouse; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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