Document Detail


Reprogramming primordial germ cells (PGC) to embryonic germ (EG) cells.
MedLine Citation:
PMID:  18770625     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this unit we describe the derivation of pluripotent embryonic germ (EG) cells from mouse primordial germ cells (PGCs) isolated from both 8.5- and 11.5-days post-coitum (dpc) embryos. Once EG cells are derived we explain how to propagate and characterize the cell lines. We introduce readers to PGCs and explain differences between PGCs and their in vitro derivatives EG cells. Finally, we also compare mouse EG cells with ES cells. This unit will be of great interest to anyone interested in PGCs or studying the behavior of cultured PGCs or the derivation of new EG cell lines.
Authors:
Gabriela Durcova-Hills; Azim Surani
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in stem cell biology     Volume:  Chapter 1     ISSN:  1938-8969     ISO Abbreviation:  Curr Protoc Stem Cell Biol     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-09-04     Completed Date:  2008-10-23     Revised Date:  2009-12-11    
Medline Journal Info:
Nlm Unique ID:  101470226     Medline TA:  Curr Protoc Stem Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit1A.3     Citation Subset:  IM    
Affiliation:
The Wellcome Trust/Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, Cambridge, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism
Animals
Antigens, CD15 / metabolism
Cell Culture Techniques / methods*
Cell Line
Cell Separation
Cryopreservation
Embryo, Mammalian / cytology*
Fluorescent Antibody Technique
Germ Cells / cytology*
Magnetics
Mice
Microspheres
Mitosis
Nuclear Reprogramming*
Octamer Transcription Factor-3 / metabolism
Polymerase Chain Reaction
Sex Determination (Analysis)
Stem Cells / cytology*
Chemical
Reg. No./Substance:
0/Antigens, CD15; 0/Octamer Transcription Factor-3; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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