| Reprogramming primordial germ cells (PGC) to embryonic germ (EG) cells. | |
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MedLine Citation:
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PMID: 18770625 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this unit we describe the derivation of pluripotent embryonic germ (EG) cells from mouse primordial germ cells (PGCs) isolated from both 8.5- and 11.5-days post-coitum (dpc) embryos. Once EG cells are derived we explain how to propagate and characterize the cell lines. We introduce readers to PGCs and explain differences between PGCs and their in vitro derivatives EG cells. Finally, we also compare mouse EG cells with ES cells. This unit will be of great interest to anyone interested in PGCs or studying the behavior of cultured PGCs or the derivation of new EG cell lines. |
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Authors:
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Gabriela Durcova-Hills; Azim Surani |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in stem cell biology Volume: Chapter 1 ISSN: 1938-8969 ISO Abbreviation: Curr Protoc Stem Cell Biol Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-09-04 Completed Date: 2008-10-23 Revised Date: 2009-12-11 |
Medline Journal Info:
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Nlm Unique ID: 101470226 Medline TA: Curr Protoc Stem Cell Biol Country: United States |
Other Details:
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Languages: eng Pagination: Unit1A.3 Citation Subset: IM |
Affiliation:
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The Wellcome Trust/Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, Cambridge, United Kingdom. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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metabolism Animals Antigens, CD15 / metabolism Cell Culture Techniques / methods* Cell Line Cell Separation Cryopreservation Embryo, Mammalian / cytology* Fluorescent Antibody Technique Germ Cells / cytology* Magnetics Mice Microspheres Mitosis Nuclear Reprogramming* Octamer Transcription Factor-3 / metabolism Polymerase Chain Reaction Sex Determination (Analysis) Stem Cells / cytology* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD15; 0/Octamer Transcription Factor-3; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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