| Reprogramming the pluripotent cell cycle: restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells. | |
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MedLine Citation:
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PMID: 20945438 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Induced pluripotent stem (iPS) cells derived from terminally differentiated human fibroblasts are reprogrammed to possess stem cell like properties. However, the extent to which iPS cells exhibit unique properties of the human embryonic stem (hES) cell cycle remains to be established. hES cells are characterized by an abbreviated G1 phase (∼ 2.5 h) and accelerated organization of subnuclear domains that mediate the assembly of regulatory machinery for histone gene expression [i.e., histone locus bodies (HLBs)]. We therefore examined cell cycle parameters of iPS cells in comparison to hES cells. Analysis of DNA synthesis [5-bromo-2'-deoxy-uridine (BrdU) incorporation], cell cycle distribution (FACS analysis and Ki67 staining) and subnuclear organization of HLBs [immunofluorescence microscopy and fluorescence in situ hybridization (FISH)] revealed that human iPS cells have a short G1 phase (∼ 2.5 h) and an abbreviated cell cycle (16-18 h). Furthermore, HLBs are formed and reorganized rapidly after mitosis (within 1.5-2 h). Thus, reprogrammed iPS cells have cell cycle kinetics and dynamic subnuclear organization of regulatory machinery that are principal properties of pluripotent hES cells. Our findings support the concept that the abbreviated cell cycle of hES and iPS cells is functionally linked to pluripotency. |
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Authors:
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Prachi N Ghule; Ricardo Medina; Christopher J Lengner; Matthew Mandeville; Meng Qiao; Zbigniew Dominski; Jane B Lian; Janet L Stein; Andre J van Wijnen; Gary S Stein |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of cellular physiology Volume: 226 ISSN: 1097-4652 ISO Abbreviation: J. Cell. Physiol. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-02-22 Completed Date: 2011-04-14 Revised Date: 2012-05-02 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 1149-56 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Wiley-Liss, Inc. |
Affiliation:
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Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle Proteins
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genetics,
metabolism Cell Line Cell Nucleus / metabolism, physiology* DNA Replication Embryonic Stem Cells / metabolism, physiology* G1 Phase* / genetics Gene Expression Regulation, Developmental Humans In Situ Hybridization, Fluorescence Induced Pluripotent Stem Cells / metabolism, physiology* Ki-67 Antigen / metabolism Kinetics Microscopy, Fluorescence Mitosis* / genetics Transcription Factors / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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P01 CA082834-11/CA/NCI NIH HHS; R01 CA082834/CA/NCI NIH HHS; R01 CA139322/CA/NCI NIH HHS; R01 CA139322-02/CA/NCI NIH HHS; R01 CA139322-03/CA/NCI NIH HHS; R01 CA139322-04/CA/NCI NIH HHS; R37 DE012528-27/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Ki-67 Antigen; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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