Document Detail


Reprogramming of liver to pancreas.
MedLine Citation:
PMID:  19089370     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Islet grafts have demonstrated that patients with diabetes would benefit greatly by beta-cell therapy. However, the paucity of available islets for transplantation as well as the immunological barriers faced in allogeneic transplantation represent a tremendous barrier to regenerative approaches to the treatment of diabetes. Here, we present a strategy and protocols to transdifferentiate developmentally related hepatocytes into beta-cells by the ectopic expression of critical beta-cell transcription factors.
Authors:
Shifaan Thowfeequ; Wan-Chun Li; Jonathan M W Slack; David Tosh
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  482     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2009  
Date Detail:
Created Date:  2008-12-17     Completed Date:  2009-02-11     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  407-18     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics
Animals
Cell Line
Cell Separation
Cells, Cultured
Fluorescent Antibody Technique
Gene Expression Regulation
Hepatocytes / cytology,  metabolism,  virology
Humans
Liver / cytology,  metabolism*
Nuclear Reprogramming*
Pancreas / cytology,  metabolism*
Rats
Reverse Transcriptase Polymerase Chain Reaction
Tissue Engineering / methods*
Transfection
Grant Support
ID/Acronym/Agency:
G0300415//Medical Research Council

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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