Document Detail

Reprogramming donor cells with oocyte extracts improves in vitro development of nuclear transfer embryos.
MedLine Citation:
PMID:  19081212     Owner:  NLM     Status:  MEDLINE    
This study investigated the effects of donor cells pretreated with oocyte extracts on in vitro development of cloned embryos. Bovine fibroblasts were exposed to immature, mature and parthenogenetic oocyte extracts respectively before nuclear transfer. The detectable expression of Oct4 and global deacetylation in the treated cells showed that extracts could reprogram fibroblasts. Although all three groups of extracts exhibited reprogramming capacity, embryo development was not compliant with reprogramming effect. Improved quality and development of blastocysts were observed only in the mature extract treated group. We demonstrated that pretreatment of donor cells with mature oocyte extract improved in vitro development of cloned embryos. Our results suggested that reprogramming donor nuclei to a state synchronized with recipient cytoplasm before nuclear transfer would be beneficial for the development of cloned embryos.
Shuang Tang; Yongsheng Wang; Dong Zhang; Yajun Gao; Yefei Ma; Baoying Yin; Jianhong Sun; Jun Liu; Yong Zhang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-31
Journal Detail:
Title:  Animal reproduction science     Volume:  115     ISSN:  1873-2232     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-08-03     Completed Date:  2009-10-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-9     Citation Subset:  IM    
Institute of Biotechnology, Northwest A&F University, Yangling, Shaanxi 712100, China.
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MeSH Terms
Actins / genetics
Blastocyst / cytology,  physiology
Cloning, Organism / methods,  veterinary
DNA Primers
Embryo Transfer
Fertilization in Vitro / veterinary
Fibroblasts / physiology*
Nuclear Transfer Techniques / veterinary*
Octamer Transcription Factor-3 / genetics
Oocytes / physiology*
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Actins; 0/DNA Primers; 0/Octamer Transcription Factor-3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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