Document Detail

Reprogramming of cell junction modules during stepwise epithelial to mesenchymal transition and accumulation of malignant features in vitro in a prostate cell model.
MedLine Citation:
PMID:  20969863     Owner:  NLM     Status:  MEDLINE    
Epithelial to mesenchymal transition (EMT) is pivotal in tumor metastasis. Our previous work reported an EMT model based on primary prostate epithelial cells (EP156T) which gave rise to cells with mesenchymal phenotype (EPT1) without malignant transformation. To promote prostate cell transformation, cells were maintained in saturation density cultures to select for cells overriding quiescence. Foci formed repeatedly following around 8 weeks in confluent EPT1 monolayers. Only later passage EPT1, but not EP156T cells of any passage, could form foci. Cells isolated from the foci were named EPT2 and formed robust colonies in soft agar, a malignant feature present neither in EP156T nor in EPT1 cells. EPT2 cells showed additional malignant traits in vitro, including higher ability to proliferate following confluence, higher resistance to apoptosis and lower dependence on exogenous growth factors than EP156T and EPT1 cells. Microarray profiling identified gene sets, many of which belong to cell junction modules, that changed expression from EP156T to EPT1 cells and continued to change from EPT1 to EPT2 cells. Our findings provide a novel stepwise cell culture model in which EMT emerges independently of transformation and is associated with subsequent accumulation of malignant features in prostate cells. Reprogramming of cell junction modules is involved in both steps.
Xi-song Ke; Wen-cheng Li; Randi Hovland; Yi Qu; Run-hui Liu; Emmet McCormack; Frits Thorsen; Jan Roger Olsen; Anders Molven; Ira Kogan-Sakin; Varda Rotter; Lars A Akslen; Anne Margrete Oyan; Karl-Henning Kalland
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-20
Journal Detail:
Title:  Experimental cell research     Volume:  317     ISSN:  1090-2422     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-11-29     Completed Date:  2011-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  234-47     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
The Gade Institute, University of Bergen, Bergen, Norway.
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MeSH Terms
Cell Culture Techniques
Cell Dedifferentiation*
Cell Line
Cell Movement
Cell Proliferation
Cell Transformation, Neoplastic / pathology*
Clone Cells
Epithelial Cells / cytology*,  pathology
Epithelial-Mesenchymal Transition*
Gene Expression Profiling
Intercellular Junctions / pathology*
Microsatellite Repeats
Prostate / cytology*,  metabolism,  pathology

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