Document Detail

Reprogramming of Xist against the pluripotent state in fusion hybrids.
MedLine Citation:
PMID:  19843582     Owner:  NLM     Status:  MEDLINE    
The fusion of somatic cells with pluripotent cells results in the generation of pluripotent hybrid cells. Because the ;memory' of somatic cells seems to be erased during fusion-induced reprogramming, genetic reprogramming is thought to be a largely unidirectional process. Here we show that fusion-induced reprogramming, which brings about the formation of pluripotent hybrids, does not always follow a unidirectional route. Xist is a unique gene in that it is reprogrammed to the state of somatic cells in fusion-induced pluripotent hybrids. In hybrids formed from the cell fusion of embryonal carcinoma cells (ECCs) with male neural stem cells (mNSCs), the Xist gene was found to be reprogrammed to the somatic cell state, whereas the pluripotency-related and tissue-specific marker genes were reprogrammed to the pluripotent cell state. Specifically, Xist is not expressed in hybrids, because the ;memory' of the somatic cell has been retained (i.e. mNSCs do not exhibit Xist expression) and that of the pluripotent cell erased (i.e. inactivation of the partially active Xist gene of ECCs, complete methylation of the Xist region). The latter phenomenon is induced by male, but not by female, NSCs.
Jeong Tae Do; Dong Wook Han; Luca Gentile; Ingeborg Sobek-Klocke; Anton Wutz; Hans R Schöler
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-20
Journal Detail:
Title:  Journal of cell science     Volume:  122     ISSN:  1477-9137     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-13     Completed Date:  2010-07-19     Revised Date:  2014-02-24    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  4122-9     Citation Subset:  IM    
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MeSH Terms
Adult Stem Cells / cytology,  metabolism*
Cell Communication*
Cell Fusion
Cell Line
DNA Methylation
Embryonal Carcinoma Stem Cells / cytology,  metabolism*
Gene Expression*
Hybrid Cells / cytology,  metabolism*
Neurons / cytology,  metabolism
Nuclear Reprogramming / genetics*
Pluripotent Stem Cells / cytology,  metabolism*
RNA, Long Noncoding
RNA, Untranslated / genetics*,  metabolism*
Sex Factors
Grant Support
087530//Wellcome Trust; G0800784//Medical Research Council
Reg. No./Substance:
0/RNA, Long Noncoding; 0/RNA, Untranslated; 0/XIST non-coding RNA

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