Document Detail


Reprogramming of human umbilical cord stromal mesenchymal stem cells for myogenic differentiation and muscle repair.
MedLine Citation:
PMID:  20665127     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human umbilical cord stromal mesenchymal stem cells (hUCS-MSCs) have the potential to differentiate into numerous cell types including epithelial cells, neurons and hepatocytes in vitro, in addition to mesenchyme-derived cells such as osteocytes, chondrocytes and adipocytes. One important property of these cells is the lack of type II major histocompatibility complex class molecules, thus allowing them to be considered as an excellent candidate for transplantations. Besides the use of 5-azacytidine as a supraphysiological inducer of myogenic transformation, no study has been published to date addressing the myogenic transformation efficiency of hUCS-MSCs by using a gene transfection strategy and/or co-culture with muscle cell lines. Here, we demonstrate the reprogramming efficiency of these cells, which differentiate into myocytes in vitro by MyoD transcription factor, the master regulator of skeletal muscle differentiation. Once induced via MyoD expression, hUCS-MSCs exhibited many cellular signs of myogenic conversion within 5 days and became capable of forming multinucleated myofibers, which exhibited all functional markers of fusion machinery such as β-catenin, neural cell adhesion molecule and M-cadherin as well as muscle cell-specific structural proteins including desmin, α-actinin, dystrophin, myosin heavy chain, and myoglobin together with muscle-specific enzyme, creatinine phosphokinase. Furthermore, programmed hUCS-MSCs were also capable of fusing with rat primary myoblasts to form heterokaryonic myotubes. Taken together, this study demonstrates the success of a novel cell reprogramming approach to be further evaluated at the in vivo level for use in restoring the defective dystrophin function as intrinsically found in the skeletal muscle fibers of Duchenne muscular dystrophy patients.
Authors:
Cetin Kocaefe; Deniz Balci; Burcu Balci Hayta; Alp Can
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stem cell reviews     Volume:  6     ISSN:  1558-6804     ISO Abbreviation:  Stem Cell Rev     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-10-04     Completed Date:  2011-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101255952     Medline TA:  Stem Cell Rev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  512-22     Citation Subset:  IM    
Affiliation:
Department of Medical Biology, Hacettepe University School of Medicine, Ankara, Turkey. kocaefe@hecettepe.edu.tr
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / genetics,  physiology*
Cells, Cultured
Humans
Immunoblotting
Immunohistochemistry
Mesenchymal Stem Cells / cytology*,  metabolism
Mice
Microscopy, Fluorescence
Muscle Cells / cytology,  metabolism
Muscle Development / genetics,  physiology*
Muscle Fibers, Skeletal / cytology,  metabolism
MyoD Protein / genetics,  metabolism
Myoblasts / cytology,  metabolism
Rats
Reverse Transcriptase Polymerase Chain Reaction
Stromal Cells / cytology*,  metabolism
Umbilical Cord / cytology*
Chemical
Reg. No./Substance:
0/MyoD Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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