| Reproducibility and efficiency testing of two sampling methods for image analysis in cytology. | |
| | |
MedLine Citation:
|
PMID: 9569966 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: Quantitative nuclear DNA analyses and nuclear morphometry have become a widely used tool in pathology. Nevertheless, studies done by different observers utilizing the same methods very often have shown different results. One of the reasons could be that different sampling methods were used. In this study two selection methods for image analyses (the consecutive selection method and the random selection method) were tested for reproducibility and efficiency. STUDY DESIGN: Using an interactive imaging analysis system, 10 cases (5 breast adenocarcinomas, 3 prostatic cancers, 1 sarcoma, 2 prostatic hyperplasias) were measured twice by using each sampling method. Median nuclear area and entropy (i.e., a statistical descriptor of DNA histograms) were the parameters calculated. RESULTS: The results showed that using the consecutive selection method, the number of individual nuclei in a cytologic sample that should be measured to obtain reproducible results was 150 for nuclear area and 110 for entropy. The corresponding figures using random systematic selection method were 80 for assessment of nuclear area and 100 for assessment of entropy. CONCLUSION: Both methods showed high reproducibility and a good correlation with each other. The mathematical idea of "plateau values," which was used to estimate the number of measurements needed, was considered a good contribution to increasing the efficiency of both methods. |
| | |
Authors:
|
F Altenstrasser; A R Weger; J Lindholm; A Gschwendter; T Mairinger |
Related Documents
:
|
20392786 - Sample size re-estimation in a breast cancer trial. 20689576 - Model of tooth morphogenesis predicts carabelli cusp expression, size, and symmetry in ... 12886276 - Effective size in simple metapopulation models. 16283866 - A comparison of self-selectivity corrections in economic evaluations and outcomes resea... 19936936 - A comparative study of uv-spectrophotometry and first-order derivative uv-spectrophotom... 15740866 - Detection of anthelmintic resistance: a comparison of mathematical techniques. |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology Volume: 20 ISSN: 0884-6812 ISO Abbreviation: Anal. Quant. Cytol. Histol. Publication Date: 1998 Apr |
Date Detail:
|
Created Date: 1998-06-10 Completed Date: 1998-06-10 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8506819 Medline TA: Anal Quant Cytol Histol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 92-6 Citation Subset: IM |
Affiliation:
|
Department of Pathology, University of Innsbruck, Austria. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Breast Neoplasms
/
pathology Cytological Techniques* Evaluation Studies as Topic Female Humans Image Processing, Computer-Assisted / methods, standards* Male Prostatic Hyperplasia / pathology Prostatic Neoplasms / pathology Reproducibility of Results Sarcoma / pathology |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Accuracy of nuclear classification in cervical smear images. Quantitative impact of computational de...
Next Document: Atomic force microscopy in effusion cytology.