Document Detail

Reproducibility of cervicovaginal ThinPrep cellularity assessment.
MedLine Citation:
PMID:  11782081     Owner:  NLM     Status:  MEDLINE    
Criteria for judging the squamous cellularity adequacy of cervicovaginal ThinPreps using cell counts in representative fields have been proposed. We compared the interobserver reproducibility in the designation of ThinPreps as satisfactory (SAT), satisfactory but limited by (SBLB), or unsatisfactory (UNS) for interpretation. Three reviewers independently assessed 145 cervicovaginal ThinPreps accessioned from 1997-1999 at the University of Kentucky cytopathology laboratory. Scant specimens were retrieved by computer search and more cellular specimens were added to make 145. Adjacent fields on a diameter including center at 10x and 40x were counted for sufficiently preserved and unobscured cells. A specimen was designated UNS if four or more fields at 10x had less than 10 squamous cells, and SBLB if four or more fields at 40x had less than five squamous cells. All three observers agreed on the adequacy designation in 76% of cases and the overall kappa was 0.73 (near excellent). Complete interobserver agreement was present in 86% (63/73) of UNS cases, 79% (33/42) of SAT, but only 47% (14/30) of SBLB slides. When SBLB and UNS categories were combined, the discrimination from the SAT category was excellent (kappa = 0.87) with pairwise k-values of 0.94-0.97. In conclusion, adjacent field cell count methods to judge ThinPrep cellularity provide near excellent interobserver reproducibility. While we have designated both UNS and SBLB adequacy categories, criteria could easily be revised to set one threshold to separate SAT and UNS ThinPreps.
Shamsa Haroon; Luis Samayoa; Donald Witzke; Diane Davey
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diagnostic cytopathology     Volume:  26     ISSN:  8755-1039     ISO Abbreviation:  Diagn. Cytopathol.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-01-08     Completed Date:  2002-02-21     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8506895     Medline TA:  Diagn Cytopathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  19-21     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Wiley-Liss, Inc.
Department of Pathology and Laboratory Medicine, University of Kentucky, Lexington, Kentucky 40536-0298, USA.
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MeSH Terms
Cell Count
Cervix Uteri / pathology
Epithelial Cells / pathology
Observer Variation
Quality Assurance, Health Care
Reproducibility of Results
Sample Size
Uterine Cervical Neoplasms / pathology*
Vaginal Smears / methods*,  standards

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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