Document Detail

Repressive effect of the phytoestrogen genistein on estradiol-induced uterine leiomyoma cell proliferation.
MedLine Citation:
PMID:  19903033     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Uterine leiomyomas are the most common gynecological benign tumor and greatly affect reproductive health and well-being. They are the predominant indication for hysterectomy in premenopausal women. Current epidemiological study reported that soy products intake is inversely associated with diseases leading to hysterectomy. Genistein is a soy-derived phytoestrogen and its inhibitory effect on leiomyoma cell proliferation is reported. In this study, we investigated the siginificant inhibitory effect of genistein on estradiol (E(2))-induced leiomyoma cells proliferation. STUDY DESIGN: The Eker rat-derived uterine leiomyoma cell line ELT-3 cells were used. Cell proliferation was assessed by counting the number of cells. The expression of estrogen receptors and peroxisome proliferator-activated receptor-gamma (PPARgamma) was evaluated by Western blot analysis. RESULTS: PPARgamma was expressed in ELT-3 cells and genistein acted as PPARgamma ligand. This inhibitory effect of genistein was attenuated by the treatment of cells with PPARgamma antagonist bisphenol A diglycidyl ether (BADGE) or GW9662. CONCLUSION: These experimental findings in vitro show that the repressive effect of genistein on E(2)-induced ELT-3 cell proliferation is through the activation of PPARgamma. Genistein may be useful as an alternative therapy for leiomyoma.
Asako Miyake; Takashi Takeda; Aki Isobe; Atsuko Wakabayashi; Fumihito Nishimoto; Ken-Ichirou Morishige; Masahiro Sakata; Tadashi Kimura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology     Volume:  25     ISSN:  1473-0766     ISO Abbreviation:  Gynecol. Endocrinol.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-11-11     Completed Date:  2010-01-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807913     Medline TA:  Gynecol Endocrinol     Country:  England    
Other Details:
Languages:  eng     Pagination:  403-9     Citation Subset:  IM    
Center for Asian Traditional Medicine, Tohoku University Graduate School of Medicine, Aoba, Sendai 980-8574, Japan.
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MeSH Terms
Apoptosis / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects*
Genistein / pharmacology,  therapeutic use*
Leiomyoma / drug therapy*
PPAR gamma / metabolism
Phytoestrogens / pharmacology,  therapeutic use*
Receptors, Estrogen / metabolism
Uterine Neoplasms / drug therapy*
Reg. No./Substance:
0/Ligands; 0/PPAR gamma; 0/Phytoestrogens; 0/Receptors, Estrogen; 446-72-0/Genistein; 50-28-2/Estradiol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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