| Repressive effect of the phytoestrogen genistein on estradiol-induced uterine leiomyoma cell proliferation. | |
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MedLine Citation:
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PMID: 19903033 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Uterine leiomyomas are the most common gynecological benign tumor and greatly affect reproductive health and well-being. They are the predominant indication for hysterectomy in premenopausal women. Current epidemiological study reported that soy products intake is inversely associated with diseases leading to hysterectomy. Genistein is a soy-derived phytoestrogen and its inhibitory effect on leiomyoma cell proliferation is reported. In this study, we investigated the siginificant inhibitory effect of genistein on estradiol (E(2))-induced leiomyoma cells proliferation. STUDY DESIGN: The Eker rat-derived uterine leiomyoma cell line ELT-3 cells were used. Cell proliferation was assessed by counting the number of cells. The expression of estrogen receptors and peroxisome proliferator-activated receptor-gamma (PPARgamma) was evaluated by Western blot analysis. RESULTS: PPARgamma was expressed in ELT-3 cells and genistein acted as PPARgamma ligand. This inhibitory effect of genistein was attenuated by the treatment of cells with PPARgamma antagonist bisphenol A diglycidyl ether (BADGE) or GW9662. CONCLUSION: These experimental findings in vitro show that the repressive effect of genistein on E(2)-induced ELT-3 cell proliferation is through the activation of PPARgamma. Genistein may be useful as an alternative therapy for leiomyoma. |
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Authors:
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Asako Miyake; Takashi Takeda; Aki Isobe; Atsuko Wakabayashi; Fumihito Nishimoto; Ken-Ichirou Morishige; Masahiro Sakata; Tadashi Kimura |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology Volume: 25 ISSN: 1473-0766 ISO Abbreviation: Gynecol. Endocrinol. Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-11-11 Completed Date: 2010-01-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8807913 Medline TA: Gynecol Endocrinol Country: England |
Other Details:
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Languages: eng Pagination: 403-9 Citation Subset: IM |
Affiliation:
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Center for Asian Traditional Medicine, Tohoku University Graduate School of Medicine, Aoba, Sendai 980-8574, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects Cell Line, Tumor Cell Proliferation / drug effects* Estradiol Female Genistein / pharmacology, therapeutic use* Leiomyoma / drug therapy* Ligands PPAR gamma / metabolism Phytoestrogens / pharmacology, therapeutic use* Rats Receptors, Estrogen / metabolism Uterine Neoplasms / drug therapy* |
| Chemical | |
Reg. No./Substance:
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0/Ligands; 0/PPAR gamma; 0/Phytoestrogens; 0/Receptors, Estrogen; 446-72-0/Genistein; 50-28-2/Estradiol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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