Document Detail

Reporting research antibody use: how to increase experimental reproducibility.
MedLine Citation:
PMID:  24358895     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Research antibodies are used in a wide range of bioscience disciplines, yet it is common to hear dissatisfaction amongst researchers with respect to their quality. Although blame is often attributed to the manufacturers, scientists are not doing all they can to help themselves. One example of this is in the reporting of research antibody use. Publications routinely lack key details, including the host species, code number and even the company who supplied the antibody. Authors also fail to demonstrate that validation of the antibodies has taken place. These omissions make it harder for reviewers to establish the likely reliability of the results and for researchers to reproduce the experiments. The scale of this problem, combined with high profile concerns about experimental reproducibility, has caused the Nature Publishing Group to include a section on antibody information in their recent Reporting Checklist for Life Science Articles. In this commentary we consider the issue of reporting research antibody use and ask what details authors should be including in their publications to improve experimental reproducibility.
Matthew A Helsby; Joe R Fenn; Andrew D Chalmers
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Publication Detail:
Type:  Journal Article     Date:  2013-07-10
Journal Detail:
Title:  F1000Research     Volume:  2     ISSN:  2046-1402     ISO Abbreviation:  F1000Res     Publication Date:  2013  
Date Detail:
Created Date:  2014-02-20     Completed Date:  2014-02-20     Revised Date:  2014-02-24    
Medline Journal Info:
Nlm Unique ID:  101594320     Medline TA:  F1000Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  153     Citation Subset:  -    
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