Document Detail

Report on the second EDNAP collaborative STR exercise. European DNA Profiling Group.
MedLine Citation:
PMID:  7868009     Owner:  NLM     Status:  MEDLINE    
The European DNA Profiling Group (EDNAP) has previously carried out collaborative exercises to determine which STR systems will produce results that can be reproduced by different laboratories. The first EDNAP exercise involving STR systems focused on different types of loci: a simple locus with six common alleles (HUMTH01) and a complex locus with > 35 alleles (ACTBP2). Generally the simpler STR system was found to be readily amenable for use across a wide range of different technologies, whereas a more complex locus presented difficulties. The second EDNAP STR exercise was intended to take the process of investigation a stage further. Some laboratories are developing automation, coupled with fluorescent methods of detection and multiplex applications, whereas others use manual methods involving visual detection techniques such as silver staining. The purpose of this exercise was to determine whether loci amenable to multiplexing with automation (as a quadruplex reaction) could also be successfully used with manual methods, either by multiplexing in duplex reactions or alternatively by using just a single pair of PCR primers.
C Kimpton; P Gill; E D'Aloja; J F Andersen; W Bar; S Holgersson; S Jacobsen; V Johnsson; A D Kloosterman; M V Lareu
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Publication Detail:
Type:  Journal Article; Multicenter Study    
Journal Detail:
Title:  Forensic science international     Volume:  71     ISSN:  0379-0738     ISO Abbreviation:  Forensic Sci. Int.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-03-28     Completed Date:  1995-03-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7902034     Medline TA:  Forensic Sci Int     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  137-52     Citation Subset:  IM    
Catholic University, Rome, Italy.
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MeSH Terms
Forensic Medicine
Genetic Techniques / standards*
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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