| Report on the second EDNAP collaborative STR exercise. European DNA Profiling Group. | |
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MedLine Citation:
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PMID: 7868009 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The European DNA Profiling Group (EDNAP) has previously carried out collaborative exercises to determine which STR systems will produce results that can be reproduced by different laboratories. The first EDNAP exercise involving STR systems focused on different types of loci: a simple locus with six common alleles (HUMTH01) and a complex locus with > 35 alleles (ACTBP2). Generally the simpler STR system was found to be readily amenable for use across a wide range of different technologies, whereas a more complex locus presented difficulties. The second EDNAP STR exercise was intended to take the process of investigation a stage further. Some laboratories are developing automation, coupled with fluorescent methods of detection and multiplex applications, whereas others use manual methods involving visual detection techniques such as silver staining. The purpose of this exercise was to determine whether loci amenable to multiplexing with automation (as a quadruplex reaction) could also be successfully used with manual methods, either by multiplexing in duplex reactions or alternatively by using just a single pair of PCR primers. |
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Authors:
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C Kimpton; P Gill; E D'Aloja; J F Andersen; W Bar; S Holgersson; S Jacobsen; V Johnsson; A D Kloosterman; M V Lareu |
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Publication Detail:
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Type: Journal Article; Multicenter Study |
Journal Detail:
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Title: Forensic science international Volume: 71 ISSN: 0379-0738 ISO Abbreviation: Forensic Sci. Int. Publication Date: 1995 Jan |
Date Detail:
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Created Date: 1995-03-28 Completed Date: 1995-03-28 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7902034 Medline TA: Forensic Sci Int Country: IRELAND |
Other Details:
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Languages: eng Pagination: 137-52 Citation Subset: IM |
Affiliation:
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Catholic University, Rome, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Forensic Medicine Genetic Techniques / standards* Humans Polymerase Chain Reaction Repetitive Sequences, Nucleic Acid* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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