Document Detail


Report on the second EDNAP collaborative STR exercise. European DNA Profiling Group.
MedLine Citation:
PMID:  7868009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The European DNA Profiling Group (EDNAP) has previously carried out collaborative exercises to determine which STR systems will produce results that can be reproduced by different laboratories. The first EDNAP exercise involving STR systems focused on different types of loci: a simple locus with six common alleles (HUMTH01) and a complex locus with > 35 alleles (ACTBP2). Generally the simpler STR system was found to be readily amenable for use across a wide range of different technologies, whereas a more complex locus presented difficulties. The second EDNAP STR exercise was intended to take the process of investigation a stage further. Some laboratories are developing automation, coupled with fluorescent methods of detection and multiplex applications, whereas others use manual methods involving visual detection techniques such as silver staining. The purpose of this exercise was to determine whether loci amenable to multiplexing with automation (as a quadruplex reaction) could also be successfully used with manual methods, either by multiplexing in duplex reactions or alternatively by using just a single pair of PCR primers.
Authors:
C Kimpton; P Gill; E D'Aloja; J F Andersen; W Bar; S Holgersson; S Jacobsen; V Johnsson; A D Kloosterman; M V Lareu
Related Documents :
19879769 - Intercomparison of rn-222 determination from groundwater.
20467989 - Evaluation of medical command and control using performance indicators in a full-scale,...
1133569 - Elimination of 137-cs and 59-fe and its relationship to metabolic rates of wild small r...
17383939 - Performance and practice in uk hospital transfusion laboratories.
1983259 - The terminal quality of life and passive or active euthanasia.
15739859 - Efficacy of a mail survey appeal for a dissertation.
11050539 - Special feature for the olympics: effects of exercise on the immune system: modificatio...
2977199 - Effect of exercise on circulating atrial natriuretic peptide and left ventricular eject...
11018439 - Force and emg power spectrum during and after eccentric and concentric fatigue.
Publication Detail:
Type:  Journal Article; Multicenter Study    
Journal Detail:
Title:  Forensic science international     Volume:  71     ISSN:  0379-0738     ISO Abbreviation:  Forensic Sci. Int.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-03-28     Completed Date:  1995-03-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7902034     Medline TA:  Forensic Sci Int     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  137-52     Citation Subset:  IM    
Affiliation:
Catholic University, Rome, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Alleles
Forensic Medicine
Genetic Techniques / standards*
Humans
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Chinese population data on three tetrameric short tandem repeat loci--HUMTHO1, TPOX, and CSF1PO--der...
Next Document:  Allele frequency distribution of four PCR-amplified loci in the Spanish population.