Document Detail

Replication timing of human telomeric DNA and other repetitive sequences analyzed by fluorescence in situ hybridization and flow cytometry.
MedLine Citation:
PMID:  11716534     Owner:  NLM     Status:  MEDLINE    
The replication timing of telomeres seems to differ between species. Yeast telomeres are late replicating, whereas limited data from very few human cell lines have indicated telomere replication throughout S phase. In the present study a series of permanent cell lines and patient samples was investigated using a flow cytometric approach for telomere length determination based on in situ hybridization using peptide nucleic acid probes and DNA staining. This method permits selective analysis of cells in specific phases of the cell cycle without perturbation of the cell cycle machinery. The timing of replication of telomeric C(3)TA(2) and T(2)AG(3) repeats was found to differ between individual samples and could precede or be concomitant with the replication of bulk DNA. Replication of the T(2)AG(3) strand seemed to occur somewhat later than that of the C(3)TA(2) strand in some samples. (GTG)(n) and other repetitive sequences generally showed a replication pattern similar to that of the bulk of DNA with slightly individual differences, whereas centromeric DNA repeats consistently replicated within a short time frame in late S phase. The apparent variability in replication timing seen for telomeric DNA might suggest individual differences in firing of replication origins.
M Hultdin; E Grönlund; K F Norrback; T Just; K Taneja; G Roos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  271     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-11-21     Completed Date:  2002-01-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  223-9     Citation Subset:  IM    
Department of Medical Biosciences, Pathology, Umeå University, Umeå, S-90187, Sweden.
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MeSH Terms
Cell Cycle / genetics*
Centromere / genetics*
DNA Replication / physiology*
Flow Cytometry
Gene Expression Regulation, Neoplastic / physiology
Hematologic Neoplasms / genetics
In Situ Hybridization, Fluorescence
Repetitive Sequences, Nucleic Acid / physiology*
S Phase / genetics
Telomere / genetics*
Time Factors
Tumor Cells, Cultured
Yeasts / genetics

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