Document Detail

Replication properties of mini-Rts1 derivatives deleted for DnaA boxes in the replication origin.
MedLine Citation:
PMID:  2550979     Owner:  NLM     Status:  MEDLINE    
Mini-Rts1 was found to be unable to replicate in a dnaA-null mutant. However, a mini-Rts1 derivative lacking entire tandem DnaA boxes in the replication origin retained the replication ability in a dnaA+ host although its copy number was about half that of the mini-Rts1 having complete DnaA boxes. Mini-Rts1cop1 that contains a high copy number mutation in repA was found to replicate more efficiently than mini-Rts1 of wild repA when DnaA boxes were deleted. In addition, the copy number of mini-Rts1cop1 without DnaA boxes increased 1.5-fold upon removal of incI iterons, whereas that of mini-Rts1 without DnaA boxes did not increase after the iterons were deleted. These indicate that the RepAcop1 protein can initiate the replication of mini-Rts1 efficiently even when DnaA boxes are absent from the origin of replication.
Y Itoh; Y Terawaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plasmid     Volume:  21     ISSN:  0147-619X     ISO Abbreviation:  Plasmid     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-10-26     Completed Date:  1989-10-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7802221     Medline TA:  Plasmid     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  242-6     Citation Subset:  IM    
Department of Bacteriology, Shinshu University School of Medicine, Matsumoto, Japan.
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MeSH Terms
Bacterial Proteins / genetics*
Base Sequence
DNA Replication*
DNA, Bacterial / genetics*
Promoter Regions, Genetic
Transformation, Genetic
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial

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