Document Detail

Replication in restenotic atherectomy tissue.
MedLine Citation:
PMID:  10996346     Owner:  NLM     Status:  MEDLINE    
Previously, we demonstrated that replication in restenotic coronary atherectomy specimens was an infrequent and modest event. In general, this data was interpreted with caution, as immunocytochemistry for the proliferating cell nuclear antigen (PCNA) was used to subjectively assess proliferation and most of the tissue specimens were resected more than 3 months after the initial interventional procedure. The purpose of the present study was to use a more sensitive method of detecting replication, in situ hybridization for histone 3 (H3) mRNA, to determine the replication profile of human directional atherectomy specimens. Restenotic directional coronary atherectomy specimens from lesions that had undergone an interventional procedure within the preceding 3 months were studied. In addition, larger atherectomy specimens from peripheral arterial lesions were assessed to ensure that pockets of replication were not being overlooked in the smaller coronary specimens. We found evidence for replication in tissue resected from 2/17 coronary and 9/12 peripheral artery restenotic lesions. In contrast, 3/11 specimens resected from primary lesions of peripheral arteries also expressed H3 mRNA. We estimated that the maximum percentage of cells that were replicating in restenotic coronary, restenotic peripheral and primary peripheral artery tissue slides to be <0.5, < or =1.2 and <0.01%, respectively. Replication was found in tissue specimens resected both early and late after a previous interventional procedure. For specimens with >15 replicating cells per slide we found high levels of focal replication. Therefore, cell replication, as assessed by the expression of H3 mRNA, was infrequent in restenotic coronary artery specimens, whereas peripheral restenotic lesions had more frequent and higher levels of replication regardless of the interval from the previous interventional procedure. For all specimens the percentage of cells that were replicating was low, however focal areas with relatively high replication indices were presented. Although replication was more abundant in restenotic lesions it does not appear to be a dominant event in the pathophysiology of restenosis.
E R O'Brien; S Urieli-Shoval; M R Garvin; D K Stewart; T Hinohara; J B Simpson; E P Benditt; S M Schwartz
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Atherosclerosis     Volume:  152     ISSN:  0021-9150     ISO Abbreviation:  Atherosclerosis     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-11-15     Completed Date:  2000-11-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  117-26     Citation Subset:  IM    
Vascular Biology Laboratory, Department of Medicine (Cardiology), University of Ottawa Heart Institute, 40 Ruskin Street, Ont., K1Y 4W7, Ottawa, Canada.
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MeSH Terms
Cell Division
Coronary Artery Disease / pathology*,  surgery
Coronary Disease / pathology*
Culture Techniques
Endothelium, Vascular / pathology
Histones / diagnostic use,  genetics
In Situ Hybridization
Middle Aged
Muscle, Smooth, Vascular / cytology,  pathology*
RNA, Messenger / analysis*
Reference Values
Sensitivity and Specificity
Grant Support
Reg. No./Substance:
0/Histones; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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