Document Detail


Replication and immunoactivity of the recombinant Peromyscus maniculatus cytomegalovirus expressing hantavirus G1 glycoprotein in vivo and in vitro.
MedLine Citation:
PMID:  16125285     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previously, we have shown that CMV isolated from deer mouse could be used in vivo and in vitro to express Sin Nombre virus (SNV) glycoprotein G1 in deer mice [Rizvanov AA, van Geelen AG, Morzunov S, et al. Generation of a recombinant cytomegalovirus for expression of a hantavirus glycoprotein. J.Virol. 2003;77(22):12203-10]. In this report, we further characterize replication of wild-type (wt) and recombinant Peromyscus CMV (PCMV) in vivo and in vitro using realtime PCR, and infectious center assays. Our findings indicate that both wt PCMV and recombinant PCMV establish persistent infections in P. maniculatus. In addition, we demonstrated that gamma irradiation of PCMV infected mice resulted in reactivation of recombinant PCMV in persistently infected mice.
Authors:
Albert A Rizvanov; Svetlana F Khaiboullina; Albert G M van Geelen; Stephen C St Jeor
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2005-08-11
Journal Detail:
Title:  Vaccine     Volume:  24     ISSN:  0264-410X     ISO Abbreviation:  Vaccine     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-19     Completed Date:  2006-02-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8406899     Medline TA:  Vaccine     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  327-34     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Nevada, Reno. Reno, NV 89557, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cytomegalovirus / genetics,  immunology*
DNA / biosynthesis,  genetics
DNA Replication
Hantavirus / genetics*,  immunology*
Peromyscus / virology*
Reverse Transcriptase Polymerase Chain Reaction
Vaccines, Synthetic / immunology
Viral Envelope Proteins / biosynthesis*,  genetics
Viral Vaccines / immunology*
Virus Replication
Whole-Body Irradiation
Grant Support
ID/Acronym/Agency:
AI45059/AI/NIAID NIH HHS; HLBI 63470/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Vaccines, Synthetic; 0/Viral Envelope Proteins; 0/Viral Vaccines; 0/glycoprotein G1, Hantavirus; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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