Document Detail

Replication of centromeric heterochromatin in mouse fibroblasts takes place in early, middle, and late S phase.
MedLine Citation:
PMID:  16231189     Owner:  NLM     Status:  MEDLINE    
The replication of eukaryotic chromosomes takes place throughout S phase, but little is known how this process is organized in space and time. Early and late replicating chromosomal domains appear to localize to distinct spatial compartments of the nucleus where DNA synthesis can take place at defined times during S phase. In general, transcriptionally active chromatin replicates early in S phase whereas transcriptionally inactive chromatin replicates later. Here we provide evidence for significant deviation from this dogma in mouse NIH3T3 cells. While the bulk pericentromeric heterochromatin replicates exclusively during mid to late S phase, centromeric DNA domains associated with constitutive kinetochore proteins are replicated throughout all stages of S phase. On an average, 12+/-4% of centromeres replicate in early S phase. Early replication of a subset of centromeres was also detected in living C2C12 murine cells. Thus, in contrast to expectation, late replication is not an obligatory feature of centromeric heterochromatin in murine cells and it does not determine their 'heterochromatic state'.
Stefanie Weidtkamp-Peters; Hans-Peter Rahn; M Cristina Cardoso; Peter Hemmerich
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-18
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  125     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-06     Completed Date:  2006-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  91-102     Citation Subset:  IM    
Institute of Molecular Biotechnology, Beutenbergstr. 11, 07745, Jena, Germany.
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MeSH Terms
3T3 Cells
Cell Nucleus / ultrastructure
Centromere / physiology*,  ultrastructure
DNA Replication / genetics
Fibroblasts / metabolism*,  ultrastructure
Fluorescent Antibody Technique
Heterochromatin / physiology*,  ultrastructure
Kinetochores / physiology,  ultrastructure
Microscopy, Fluorescence
Mitosis / physiology*
Plasmids / genetics
S Phase / physiology*
Reg. No./Substance:

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