Document Detail


Replication of Spodoptera exigua nucleopolyhedrovirus in permissive and non-permissive lepidopteran cell lines.
MedLine Citation:
PMID:  10358729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Spodoptera exigua multinucleocapsid nucleopolyhedrovirus (SeMNPV) was inoculated to eight lepidopteran cell lines derived from Spodoptera exigua (Se301), Spodoptera frugiperda (SF21AEII), Spodoptera littoralis (CLS-79), Spodoptera litura (SpLi-221), Pseudaletia separata (LeSe-11), Trichoplusia ni (hi-5), Plutella xylostella (PXL/C) and Bombyx mori (BmN4). The productive infection of SeMNPV was observed only in Se301 cells. However, a dot-blot hybridization analysis revealed that SeMNPV DNA replicated in five non-permissive cell lines: SF21AEII, CLS-79, SpLi-221, hi-5 and BmN4. In addition, the virus-infected hi-5 and BmN4 cells displayed morphological changes. In contrast, CLS-79 cells inoculated with SeMNPV showed membrane blebbing at 20 hrs post inoculation (p.i.) and fragmentation of genomic DNA. All that indicated that the infected CLS-79 cells underwent apoptosis. These findings indicate that the SeMNPV replication was restricted at various points in dependence upon each cell line.
Authors:
T Yanase; C Yasunaga; T Kawarabata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta virologica     Volume:  42     ISSN:  0001-723X     ISO Abbreviation:  Acta Virol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-06-24     Completed Date:  1999-06-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370401     Medline TA:  Acta Virol     Country:  CZECH REPUBLIC    
Other Details:
Languages:  eng     Pagination:  293-8     Citation Subset:  IM    
Affiliation:
Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis
Bombyx
Cell Line / virology
DNA, Viral / analysis
Nucleopolyhedrovirus / growth & development*,  isolation & purification
Species Specificity
Spodoptera
Time Factors
Virus Replication
Chemical
Reg. No./Substance:
0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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