| Replication of the R6K plasmid during the Escherichia coli cell cycle. | |
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MedLine Citation:
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PMID: 1732198 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cell-cycle replication pattern of the R6K plasmid has been investigated by using the membrane-elution technique to produce cells labelled at different times during the division cycle and scintillation counting for quantitative analysis of radioactive plasmid DNA. The high-copy plasmid R6K replicates exponentially in a cell-cycle-independent manner. A mini-R6K plasmid deleted for the ori alpha origin of replication also replicates, exponentially in a cell-cycle-independent manner. |
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Authors:
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J D Keasling; B O Palsson; S Cooper |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 174 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1992 Feb |
Date Detail:
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Created Date: 1992-02-27 Completed Date: 1992-02-27 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1060-2 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, University of Michigan, Ann Arbor 48109-2136. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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physiology* DNA Replication* Escherichia coli / physiology* Plasmids / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM44022-01A1/GM/NIGMS NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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